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通过大肠杆菌噬菌体P1将基因转移至粘细菌

Gene transfer to myxobacterium by Escherichia coli phage P1.

作者信息

Kaiser D, Dworkin M

出版信息

Science. 1975 Feb 21;187(4177):653-4. doi: 10.1126/science.803710.

DOI:10.1126/science.803710
PMID:803710
Abstract

Myxococcus xanthus is a bacterium with an interest for studies of development because it has an organized multicellular phase in its life cycle. Bacteriophage Pl can adsorb to M. xanthus and inject its DNA into this organism despite the wide taxonomic gap separating myxococcus from Escherichia coli, the source of Pl. A specialized transducing derivative of Pl, called PlCM, can carry a gene for chloramphenicol resistance from E. coli into M. xanthus and generate unstable drug-resistant strains.

摘要

黄色粘球菌是一种在发育研究方面具有研究价值的细菌,因为它在生命周期中具有有组织的多细胞阶段。尽管粘球菌与噬菌体P1的来源大肠杆菌在分类学上差距很大,但噬菌体P1仍能吸附到黄色粘球菌上并将其DNA注入该生物体。P1的一种特殊转导衍生物,称为P1CM,可以将来自大肠杆菌的氯霉素抗性基因携带到黄色粘球菌中,并产生不稳定的耐药菌株。

相似文献

1
Gene transfer to myxobacterium by Escherichia coli phage P1.通过大肠杆菌噬菌体P1将基因转移至粘细菌
Science. 1975 Feb 21;187(4177):653-4. doi: 10.1126/science.803710.
2
Transduction of Myxococcus virescens by coliphage P1CM: generation of plasmids containing both phage and Myxococcus genes.用大肠杆菌噬菌体P1CM转导绿粘球菌:产生含有噬菌体和绿粘球菌基因的质粒。
J Gen Microbiol. 1978 Jul;107(1):73-83. doi: 10.1099/00221287-107-1-73.
3
Extrachromosomal DNA in chloramphenicol resistant myxococcus strains.氯霉素抗性粘球菌菌株中的染色体外DNA。
J Gen Microbiol. 1976 Mar;93(1):63-8. doi: 10.1099/00221287-93-1-63.
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Transfer of drug resistance to myxococcus from bacteria carrying drug-resistance factors.耐药性从携带耐药因子的细菌转移至黏球菌。
J Gen Microbiol. 1975 Apr;87(2):198-210. doi: 10.1099/00221287-87-2-198.
5
Genetic analysis of Myxococcus xanthus and isolation of gene replacements after transduction under conditions of limited homology.黄色粘球菌的遗传分析及在有限同源性条件下转导后基因替代物的分离
J Bacteriol. 1986 Aug;167(2):744-8. doi: 10.1128/jb.167.2.744-748.1986.
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Transduction of R factors with phage P1 from recombination-deficient Escherichia coli.
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Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses.噬菌体温和噬菌体P1介导的克隆DNA从大肠杆菌到黄色粘球菌的转导:用于互补和重组分析。
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Lysogenic conversion of Pasteurella by Escherichia coli bacteriophage P1 CM.大肠杆菌噬菌体P1 CM介导的巴斯德氏菌溶原性转变
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Plaque-forming transducing bacteriophage P1 derivatives and their behaviour in lysogenic conditions.形成噬菌斑的转导噬菌体P1衍生物及其在溶原状态下的行为。
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Is the IS1-flanked r-determinant of the R plasmid NR1 a transposon?R质粒NR1中IS1侧翼的r决定子是转座子吗?
J Gen Microbiol. 1981 Oct;126(2):413-25. doi: 10.1099/00221287-126-2-413.

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