Parish J H
J Gen Microbiol. 1975 Apr;87(2):198-210. doi: 10.1099/00221287-87-2-198.
Resistance to chloramphenicol was successfully transferred from strains of Escherichia coli carrying R factors representative of compatibility groups F, W, S and N to strains of Myxococcus xanthus and M. fulvus. Resistance to kanamycin was transferred from an R factor in group S, and to neomycin from an R factor of group P. Myxobacterial strains differed in their capacity to take up the resistances and also in the stability of the resistance character. strains of M. fulvus were obtained that acquired resistance to chloramphenicol without exposure to R plus eubacterial strains. Cell-free preparations of all the chloramphenicol-resistant strains catalysed the acetylation of the drug. Cholramphenicol resistance was successfully transferred from the presumed R plus strains of Myxococcus and also from the spontaneously occurring chloramphenicol-resistant M. fulvus to other Myxococcus strains. Moreover, recombinants resistant to both rifampicin and 5-fluorouracil were obtained, though infrequently, by mixing Myococcus strains resistant to rifampicin and chloramphenicol with other myxococci resistant to 5-fluorouracil, both when the chloramphenicol resistance was derived from S-a (group W) and when it was the endogenous M fulvus resistance. Thus it appears that S-a and a new chloramphenicol resistance factor from M. fulvus will mobilize a chromosomal genetic marker in Myxococcus.
携带代表相容性群F、W、S和N的R因子的大肠杆菌菌株中的氯霉素抗性成功转移到了黄色粘球菌和褐黄粘球菌菌株中。卡那霉素抗性从S组的一个R因子转移而来，新霉素抗性从P组的一个R因子转移而来。粘细菌菌株在吸收抗性的能力以及抗性特征的稳定性方面存在差异。获得了未接触R加真细菌菌株就获得氯霉素抗性的褐黄粘球菌菌株。所有氯霉素抗性菌株的无细胞制剂都催化了药物的乙酰化。氯霉素抗性成功地从假定的粘球菌R加菌株以及自发产生的氯霉素抗性褐黄粘球菌转移到了其他粘球菌菌株。此外，通过将对利福平及氯霉素有抗性的粘球菌菌株与对5-氟尿嘧啶有抗性的其他粘球菌混合，当氯霉素抗性源自S-a（W组）以及当它是内源性褐黄粘球菌抗性时，虽然频率不高，但获得了对利福平和5-氟尿嘧啶都有抗性的重组体。因此，似乎S-a和来自褐黄粘球菌的一个新的氯霉素抗性因子将在粘球菌中调动一个染色体遗传标记。
