Lyons A J, Pridham T G, Rogers R F
Appl Microbiol. 1975 Feb;29(2):249-9.
Mesophilic Actinomycetales were isolated from whole corn, brewers grits, and break flour received from three different mills. In addition, strains were isolated from high-moisture (27 per cent) field corn; high-moisture, silo-stored corn (untreated); and high-moisture corn treated with ammonia, ammonium isobutyrate, or propionic-acetic acid. According to standard techniques, 139 strains were extensively characterized and 207 additional strains were partially characterized. On the basis of these characterizations, the streptomycete strains were identified by both the systems of Pridham et al. and Hütter because these systems are rapid and accurate. In general, only Streptomyces griseus (Krainsky) Waksman and Henrici was isolated from high-moisture whole corn (treated or untreated) except from grain exposed to ammonium isobutyrate. Strains isolated from high-moisture corn subjected to that treatment represented both S. griseus and S. albus (Rossi Doria) Waksman and Henrici. The strains isolated from corn and corn products from the three mills were identified with a number of streptomycete species. Of all Actinomycetales isolated, only three were not streptomycetes--two from brewer's grits and one from break flour.
嗜温放线菌目细菌是从来自三家不同工厂的整粒玉米、啤酒厂粗粒和粗碎粉中分离得到的。此外，还从高水分（27%）的田间玉米、高水分、未处理的青贮玉米以及用氨、异丁酸铵或丙酸 - 乙酸处理过的高水分玉米中分离出了菌株。按照标准技术，对139株菌株进行了全面鉴定，另外207株菌株进行了部分鉴定。基于这些鉴定结果，链霉菌菌株采用Pridham等人和Hütter的系统进行鉴定，因为这些系统快速且准确。一般来说，除了暴露于异丁酸铵的谷物外，从高水分整粒玉米（处理过或未处理）中仅分离出灰色链霉菌（Krainsky）Waksman和Henrici。从经过该处理的高水分玉米中分离出的菌株包括灰色链霉菌和白色链霉菌（Rossi Doria）Waksman和Henrici。从这三家工厂的玉米和玉米产品中分离出的菌株被鉴定为多种链霉菌物种。在所有分离出的放线菌目中，只有三株不是链霉菌——两株来自啤酒厂粗粒，一株来自粗碎粉。
