Ahn S S, Williams D E, Thye D A, Cheng K Q, Lee D A
Department of Surgery-Vascular Section, UCLA Center for the Health Sciences, Los Angeles 90024.
J Vasc Surg. 1994 Aug;20(2):202-8. doi: 10.1016/0741-5214(94)90007-8.
Perigraft seroma is a rare complication of reconstructive vascular surgery characterized by a clear, sterile fluid collection confined within a fibrous pseudomembrane around a prosthetic graft. The exact cause of this disease is unknown but involves failure of surrounding connective tissue to incorporate the graft. To understand why this occurs, we studied sera from patients with perigraft seroma for their effect on human fibroblasts. Sera from control subjects, patients with uninfected prosthetic grafts, and patients with prosthetic grafts were tested for comparison.
Fibroblast growth was measured by radioactive thymidine uptake and hexosaminidase colorimetric cell proliferation assays. We fractionated sera with gel filtration columns and measured each fraction's effect on fibroblast growth.
Serum samples from patients with perigraft seroma inhibited fibroblast growth in a dose-dependent manner. In contrast, a postseroma sample, normal human sera, and sera from patients with infected and uninfected grafts showed no significant fibroblast inhibition. An inhibitory factor with a molecular weight of 2000 d was isolated from serum of patients with perigraft seroma.
Perigraft seroma is associated with a fibroblast inhibitor with a molecular weight of 2000 d. Further identification and characterization of this protein may lead to clinical applications in preventing and treating perigraft seroma.
移植周围血清肿是重建性血管手术中一种罕见的并发症，其特征为在人工血管周围的纤维假膜内有清亮、无菌的液体聚集。该病的确切病因尚不清楚，但与周围结缔组织未能包裹人工血管有关。为了解其发生原因，我们研究了移植周围血清肿患者的血清对人成纤维细胞的影响。作为对照，我们检测了健康受试者、未感染人工血管患者以及感染人工血管患者的血清。
通过放射性胸苷摄取和己糖胺酶比色法细胞增殖试验来检测成纤维细胞的生长情况。我们用凝胶过滤柱对血清进行分级分离，并检测各分级对成纤维细胞生长的影响。
移植周围血清肿患者的血清样本以剂量依赖方式抑制成纤维细胞生长。相比之下，血清肿后样本、正常人血清以及感染和未感染人工血管患者的血清均未显示出明显的成纤维细胞抑制作用。从移植周围血清肿患者血清中分离出一种分子量为2000 d的抑制因子。
移植周围血清肿与一种分子量为2000 d的成纤维细胞抑制剂有关。对该蛋白的进一步鉴定和特性分析可能会在移植周围血清肿的预防和治疗方面带来临床应用。
