Vandergrifft E V, Swiderski C E, Horohov D W
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70808.
Vet Immunol Immunopathol. 1994 Apr;40(4):379-84. doi: 10.1016/0165-2427(94)90047-7.
We have cloned equine interleukin 4 (IL-4) cDNA using the polymerase chain reaction (PCR) and primers based on the human IL-4 sequence. The cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC). The cloned PCR product shares extensive homology ith IL-4 sequences from other species.
我们利用聚合酶链反应（PCR）和基于人白细胞介素4（IL-4）序列的引物，克隆了马白细胞介素4（IL-4）的互补DNA（cDNA）。该cDNA是从经丝裂原刺激的马外周血单个核细胞（PBMC）中扩增得到的。克隆的PCR产物与其他物种的IL-4序列具有广泛的同源性。
