Hopkins J D, Clements M, Syvanen M
J Bacteriol. 1983 Jan;153(1):384-9. doi: 10.1128/jb.153.1.384-389.1983.
We have used a papillation screening technique to isolate mutations that increase the precise excision of insertion elements. The three mutations isolated stimulated precise excision of Tn5, Tn10, and the IS elements. They had a large, 20- to 600-fold, effect on excision of Tn5 at various chromosomal sites. The varied stimulation for different Tn5 insertions showed that the mutations altered the relationship between a precise excision activity and the chromosomal sequence flanking an inserted Tn5. A much smaller stimulation was observed for insertions on the plasmid F'128. The stimulation was recA independent. The mutations also reduced the rate of production of bacteriophage Mu progeny. The mutations were mapped by two- and three-factor crosses with closely linked Tn10 insertions. They defined the uup locus, located at 21.3 min on the Escherichia coli map, next to pyrD.
我们使用了一种乳头瘤筛选技术来分离能增加插入元件精确切除的突变。分离出的三个突变刺激了Tn5、Tn10和IS元件的精确切除。它们对不同染色体位点的Tn5切除有很大影响，达到20至600倍。对不同Tn5插入的不同刺激表明，这些突变改变了精确切除活性与插入的Tn5侧翼染色体序列之间的关系。在质粒F'128上的插入观察到的刺激要小得多。这种刺激不依赖recA。这些突变还降低了噬菌体Mu子代的产生率。通过与紧密连锁的Tn10插入进行双因子和三因子杂交对这些突变进行了定位。它们确定了uup位点，位于大肠杆菌图谱上21.3分钟处，紧挨着pyrD。
