Kuo S H, Lai M Y, Liu Y R, Lee Y T, Chen D S, Lee C S, Hsu H C
Department of Clinical Pathology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Anal Quant Cytol Histol. 1994 Apr;16(2):153-8.
The nuclear area and DNA content between tumor and nontumor portions of hepatocellular carcinoma (HCC) were compared. Thirty pairs of imprint smears were made from surgical specimens and stained with a modified Feulgen reaction. Two hundred nuclei were scanned with a microdensitometer at a wavelength of 550 nm. DNA histograms were plotted with lymphocytes as the internal control. Nuclear areas of 200 cells in each case were measured with Minimagiscan image analyzer. Hyperploidy (DNA index > 1.1) was 76.7% and 53.3% in tumor and nontumor portions, respectively. The S-phase fraction value was 7.9 +/- 3.5% (+/- SD) and 4.1 +/- 2.4% in tumor and nontumor portions of HCC (P < .001). The mean nuclear area of the tumor portion was significantly larger than that of its corresponding nontumor portion (232.9 +/- 68.1 vs. 137.6 +/- 15.5 microns2, P < .001). Marked anisonucleosis was found in both the tumor and nontumor portions of HCC. The mean coefficient of variation of mean nuclear areas was 27.8 +/- 10.4% and 22.9 +/- 4.9% in the tumor and nontumor portions, respectively (P < .05). DNA ploidy correlated well with mean nuclear area in the tumor portions (r = .730) but not in the nontumor ones. The results indicate that the three most important cytologic criteria of malignant cells--hyperchromatism, nuclear enlargement and anisonucleosis--are evident in both the tumor and nontumor portions of HCC.
比较了肝细胞癌（HCC）肿瘤部分与非肿瘤部分的核面积和DNA含量。从手术标本制作了30对印片涂片，并用改良的Feulgen反应染色。用微密度计在550nm波长下扫描200个细胞核。以淋巴细胞作为内对照绘制DNA直方图。用Minimagiscan图像分析仪测量每种情况下200个细胞的核面积。肿瘤部分和非肿瘤部分的超二倍体（DNA指数>1.1）分别为76.7%和53.3%。HCC肿瘤部分和非肿瘤部分的S期分数值分别为7.9±3.5%（±标准差）和4.1±2.4%（P<.001）。肿瘤部分的平均核面积明显大于其相应的非肿瘤部分（232.9±68.1对137.6±15.5μm2，P<.001）。在HCC的肿瘤部分和非肿瘤部分均发现明显的核大小不均。肿瘤部分和非肿瘤部分平均核面积的平均变异系数分别为27.8±10.4%和22.9±4.9%（P<.05）。DNA倍性与肿瘤部分的平均核面积相关性良好（r=.730），但与非肿瘤部分无关。结果表明，恶性细胞的三个最重要的细胞学标准——核深染、核增大和核大小不均——在HCC的肿瘤部分和非肿瘤部分均很明显。
