Koomen G C, Betjes M G, Zemel D, Krediet R T, Hoek F J
Department of Clinical Chemistry, University of Amsterdam, The Netherlands.
Perit Dial Int. 1994;14(2):132-6.
The local production of cancer antigen (CA) 125 in the peritoneal cavity of 14 continuous ambulatory peritoneal dialysis patients was studied. In addition, the relationship between the concentration of mesothelial cells and CA 125 in the peritoneal dialysate effluent was examined. The median results and ranges were as follows: plasma CA 125 14 U/mL (range 10-23), dialysate CA 125 18 U/mL (range 5.2-76), dialysate/plasma ratio 1.9 (range 0.61-5.4), and number of mesothelial cells 400/mL (range 10-5000). Peritoneal concentrations of mesothelial cells and CA 125 were positively correlated (r = 0.50, p < 0.01). Using a monoclonal antibody, CA 125-positive cells were found in the cytospin preparations of the cells of dialysis effluents. All these CA 125 positive cells were also positive for cytokeratin used as a mesothelial cell marker. In vitro experiments using mesothelial cells in monolayers showed a linear increase in CA 125 concentration both in time and in relation to the number of mesothelial cells. From these experiments a production rate of 24 U/hour/10(6) cells could be calculated. It is therefore concluded that CA 125 is locally produced in the peritoneal cavity during CAPD and that the mesothelial cells are the major source of this CA 125.
研究了14例持续性非卧床腹膜透析患者腹腔内癌抗原（CA）125的局部产生情况。此外，还检测了腹膜透析流出液中间皮细胞浓度与CA 125之间的关系。中位数结果及范围如下：血浆CA 125为14 U/mL（范围10 - 23），透析液CA 125为18 U/mL（范围5.2 - 76），透析液/血浆比值为1.9（范围0.61 - 5.4），间皮细胞数量为400/mL（范围10 - 5000）。腹膜中间皮细胞浓度与CA 125呈正相关（r = 0.50，p < 0.01）。使用单克隆抗体，在透析流出液细胞的细胞涂片制剂中发现了CA 125阳性细胞。所有这些CA 125阳性细胞对用作间皮细胞标志物的细胞角蛋白也呈阳性。使用单层间皮细胞的体外实验表明，CA 125浓度随时间以及与间皮细胞数量均呈线性增加。从这些实验中可以计算出产生率为24 U/小时/10(6)个细胞。因此得出结论，在持续性非卧床腹膜透析期间，CA 125在腹腔内局部产生，且间皮细胞是这种CA 125的主要来源。
