Shirshev S V
Biull Eksp Biol Med. 1993 May;115(5):497-9.
The chorionic gonadotropin (Cg) effects on the ability of intact and mitogen-activated splenocytes to form the adoptive immune response have been studied in a short-term cell culture. It has been established, that an hour incubation of non-fractionated spleen cells with CG (40 or 200 IU) leads to plaque-forming cell (PFC) suppression. The culture, deprived of macrophages, does not react on the hormone depressive effects, but its low dose, on the contrary, stimulates the PFC formation. Lipopolysaccharide addition into the culture of non-fractionated splenocytes does not influence the PFC level, while the CG addition leads to intensification of PFC formation processes. In the cell culture, deprived of macrophages, hormone abolishes the immunostimulating effect of lipopolysaccharide. The simultaneous addition of non-fractionated CG splenocytes and monoclonal anti-IgM antibodies does not influence the immunostimulating effect of mitogenic antibodies. The possible mechanisms of CG effect and the problem of hormonal immunocompetent cell regulation, depending on their functional activity are under consideration.
在短期细胞培养中研究了绒毛膜促性腺激素（Cg）对完整的和丝裂原激活的脾细胞形成过继免疫反应能力的影响。已证实，将未分级的脾细胞与CG（40或200国际单位）孵育1小时会导致空斑形成细胞（PFC）受到抑制。去除巨噬细胞的培养物对激素的抑制作用没有反应，但低剂量的激素反而会刺激PFC的形成。向未分级脾细胞培养物中添加脂多糖不会影响PFC水平，而添加CG会导致PFC形成过程增强。在去除巨噬细胞的细胞培养物中，激素会消除脂多糖的免疫刺激作用。同时添加未分级的CG脾细胞和单克隆抗IgM抗体不会影响促有丝分裂抗体的免疫刺激作用。目前正在探讨CG作用的可能机制以及激素对免疫活性细胞的调节问题，这取决于它们的功能活性。
