Shirshev S V, Kevorkov N N
Mol Biol (Mosk). 1993 May-Jun;27(3):500-6.
The mechanisms of the molecular action of chorionic gonadotropin (CG) at different doses have been studied in splenocyte culture on the level of secondary messengers. It has been demonstrated that in the dose of 40 IU CG suppresses the splenocyte ability to form plaque-forming cells (PFC) only in the presence of macrophages through the eicosanoid system. The dose of 200 IU produced an analogous effect using Ca2+ as the secondary messenger. Transduction of the hormonal signal, leading to immunosuppression does not depend on the inositide system. It is established that in a low dose CG selectively stimulates PFC formation on the condition that there are no macrophages in the cell culture. This effect depends on Ca2+ and inositol phosphates, which are synergists. The dose of 200 IU in the absence of macrophages does not show an immunomodulating effect. We propose the presence of high-affinity receptors for CG on macrophages an B-lymphocytes and low-affinity receptor on T-lymphocyte suppressors, which are associated with "slow" Ca2+ channels.
在脾细胞培养中，已在第二信使水平研究了不同剂量绒毛膜促性腺激素（CG）的分子作用机制。结果表明，40国际单位剂量的CG仅在巨噬细胞存在的情况下，通过类花生酸系统抑制脾细胞形成空斑形成细胞（PFC）的能力。200国际单位的剂量以Ca2+作为第二信使产生了类似的效果。导致免疫抑制的激素信号转导不依赖于肌醇磷脂系统。已确定，在低剂量时，CG在细胞培养中无巨噬细胞的条件下选择性刺激PFC形成。这种作用取决于Ca2+和肌醇磷酸，它们是协同剂。在无巨噬细胞的情况下，200国际单位的剂量未显示出免疫调节作用。我们提出，巨噬细胞和B淋巴细胞上存在CG的高亲和力受体，T淋巴细胞抑制细胞上存在低亲和力受体，这些受体与“缓慢”Ca2+通道相关。
