Fava F, Di Gioia D, Bignami A, Marchetti L
Department of Applied Chemistry and Material Science, University of Bologna, Italy.
Chemosphere. 1994 Jul;29(1):39-46. doi: 10.1016/0045-6535(94)90087-6.
A strain of Pseudomonas fluorescens, designed CP30, was capable of co-metabolizing 3-chlorobenzoic acid via chlorocatechols, thus producing a polychlorocatechol black pigment. The pigment production was found to be enzyme-mediated in P. fluorescens cultures; however, the results of the autoxidation studies suggested that a large amount of pigment could derive from the simple autoxidation of the chlorocatechols accumulated in the CP30 culture during the 3-chlorobenzoate degradation. The polymerization of chlorocatechols should reduce the toxicity of the culture and generally resulted in a large production of a water-insoluble (i.e. a easily removable) pigment with the release a large amount of chloride ions. Both 3-chlorobenzoate degradation and pigment production were stimulated and inhibited in the same way by high and low pH values, respectively, in CP30 cultures; the culture conditions which permitted an extensive 3-chlorobenzoate degradation with the lowest pigment production were not identified. However, on the basis of the detoxification property of the pigment production process, high pH and dissolved oxygen concentrations appears to be the most suitable culture conditions for an enhanced detoxicant treatment of 3-clorobenzoate-contaminated waste water.
一种荧光假单胞菌菌株，编号为CP30，能够通过氯代儿茶酚对3 - 氯苯甲酸进行共代谢，从而产生一种聚氯代儿茶酚黑色色素。在荧光假单胞菌培养物中发现色素产生是由酶介导的；然而，自氧化研究结果表明，大量色素可能源自3 - 氯苯甲酸酯降解过程中CP30培养物中积累的氯代儿茶酚的简单自氧化。氯代儿茶酚的聚合应降低培养物的毒性，并且通常会大量产生水不溶性（即易于去除）的色素，并释放大量氯离子。在CP30培养物中，高pH值和低pH值分别以相同的方式刺激和抑制3 - 氯苯甲酸酯的降解和色素的产生；未确定允许大量降解3 - 氯苯甲酸酯且色素产生最少的培养条件。然而，基于色素产生过程的解毒特性，高pH值和溶解氧浓度似乎是增强对受3 - 氯苯甲酸酯污染废水进行解毒处理的最合适培养条件。
