Brummel T J, Twombly V, Marqués G, Wrana J L, Newfeld S J, Attisano L, Massagué J, O'Connor M B, Gelbart W M
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Cell. 1994 Jul 29;78(2):251-61. doi: 10.1016/0092-8674(94)90295-x.
The dpp/BMP family of TGF beta-related factors controls numerous events in pattern formation and morphogenesis. How these polypeptide signals are received and transduced by target cells is largely unknown. We combine molecular and genetic approaches to establish that the Drosophila saxophone (sax) gene encodes a dpp receptor. We compare the structural properties and expression patterns of sax with a second dpp receptor encoded by the thick veins (tkv) gene. While the sax gene is expressed ubiquitously, tkv is expressed in a highly localized and dynamic pattern during development. Some, but not all, of the tkv expression pattern parallels that of dpp. Ubiquitous expression of a tkv transgene rescues both tkv and sax loss-of-function mutations. Thus, there is at least partial functional overlap of the sax and tkv receptors in vivo. We consider these observations in terms of possible ligand-receptor interactions during Drosophila development.
与转化生长因子β相关的dpp/BMP家族因子控制着模式形成和形态发生中的众多事件。这些多肽信号如何被靶细胞接收和转导在很大程度上尚不清楚。我们结合分子和遗传学方法来确定果蝇萨克斯管（sax）基因编码一种dpp受体。我们将sax的结构特性和表达模式与由粗脉（tkv）基因编码的第二种dpp受体进行比较。虽然sax基因在全身表达，但tkv在发育过程中以高度局部化和动态的模式表达。tkv的部分（而非全部）表达模式与dpp的表达模式平行。tkv转基因的全身表达挽救了tkv和sax功能丧失突变。因此，sax和tkv受体在体内至少存在部分功能重叠。我们根据果蝇发育过程中可能的配体-受体相互作用来考虑这些观察结果。
