Letsou A, Arora K, Wrana J L, Simin K, Twombly V, Jamal J, Staehling-Hampton K, Hoffmann F M, Gelbart W M, Massagué J
Department of Human Genetics, University of Utah, Salt Lake City 84112.
Cell. 1995 Mar 24;80(6):899-908. doi: 10.1016/0092-8674(95)90293-7.
Signaling by TGF beta-related factors requires ligand-induced association between type I and type II transmembrane serine/threonine kinases. In Drosophila, the saxophone (sax) and thick veins (tkv) genes encode type I receptors that mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta-type factors. In this report, we demonstrate that the Drosophila punt gene encodes atr-II, a previously described type II receptor that on its own is able to bind activin but not BMP2, a vertebrate ortholog of dpp. Mutations in punt produce phenotypes similar to those exhibited by tkv, sax, and dpp mutants. Furthermore, punt will bind BMP2 in concert with tkv or sax, forming complexes with these receptors. We suggest that punt functions as a type II receptors for dpp and propose that BMP signaling in vertebrates may also involve sharing of type II receptors by diverse ligands.
转化生长因子β相关因子的信号传导需要配体诱导的I型和II型跨膜丝氨酸/苏氨酸激酶之间的结合。在果蝇中，萨克斯风（sax）和粗脉（tkv）基因编码I型受体，介导由骨形态发生蛋白（BMP）亚组的转化生长因子β型因子中的一种——截瘫蛋白（dpp）所引发的信号传导。在本报告中，我们证明果蝇的punt基因编码atr-II，这是一种先前描述的II型受体，其自身能够结合激活素，但不能结合dpp的脊椎动物直系同源物BMP2。punt基因的突变产生的表型与tkv、sax和dpp突变体所表现出的表型相似。此外，punt将与tkv或sax协同结合BMP2，与这些受体形成复合物。我们认为punt作为dpp的II型受体发挥作用，并提出脊椎动物中的BMP信号传导可能也涉及多种配体对II型受体的共享。
