Xie T, Finelli A L, Padgett R W
Waksman Institute, Rutgers University, Piscataway, NJ 08855-0759.
Science. 1994 Mar 25;263(5154):1756-9. doi: 10.1126/science.8134837.
The Drosophila decapentaplegic (dpp) gene encodes a transforming growth factor-beta (TGF-beta)-like protein that plays a key role in several aspects of development. Transduction of the DPP signal was investigated by cloning of serine-threonine kinase transmembrane receptors from Drosophila because this type of receptor is specific for the TGF-beta-like ligands. Here evidence is provided demonstrating that the Drosophila saxophone (sax) gene, a previously identified female sterile locus, encodes a TGF-beta-like type I receptor. Embryos from sax mothers and dpp embryos exhibit similar mutant phenotypes during early gastrulation, and these two loci exhibit genetic interactions, which suggest that they are utilized in the same pathway. These data suggest that sax encodes a receptor for dpp.
果蝇的“十五体瘫”（dpp）基因编码一种转化生长因子-β（TGF-β）样蛋白，该蛋白在发育的多个方面发挥关键作用。通过克隆果蝇的丝氨酸 - 苏氨酸激酶跨膜受体来研究DPP信号的转导，因为这种类型的受体对TGF-β样配体具有特异性。本文提供的证据表明，果蝇的“萨克斯管”（sax）基因，一个先前确定的雌性不育基因座，编码一种TGF-β样I型受体。来自sax母体的胚胎和dpp胚胎在原肠胚形成早期表现出相似的突变表型，并且这两个基因座表现出遗传相互作用，这表明它们在同一途径中发挥作用。这些数据表明sax编码dpp的受体。
