Kim S H, Ryu H, Kang C W, Kim S Z, Seul K H, Cho K W
Department of Physiology, Jeonbug National University Medical School, Republic of Korea.
Gen Comp Endocrinol. 1994 Apr;94(1):151-6. doi: 10.1006/gcen.1994.1068.
Synthesis and secretion of atrial natriuretic peptides (ANPs) are not confined to the atrium, but are also present in other tissues. Recently, we have found synthesis of ANP in the eggs of several vertebrate animals. The present study was undertaken to determine whether immunoreactive ANP (irANP) is present in the egg of an invertebrate, the silkworm (Bombyx mori L.). The serial dilution curve of egg extracts of silkworm was parallel to the standard curve of atriopeptin III. Analysis of ANP immunoreactivity by gel filtration chromatography and reverse-phase HPLC showed that the major immunoreactivity corresponded to rat proANP. The semipurified irANP of egg extracts produced a dose-dependent relaxation on rat aortic strips, which was blocked by preincubation with anti-ANP antiserum. Therefore, we suggest that ANP is synthesized in the silkworm egg.
心房利钠肽（ANPs）的合成与分泌并不局限于心房，在其他组织中也有存在。最近，我们在几种脊椎动物的卵中发现了ANP的合成。本研究旨在确定无脊椎动物家蚕（Bombyx mori L.）的卵中是否存在免疫反应性ANP（irANP）。家蚕卵提取物的系列稀释曲线与心房肽III的标准曲线平行。通过凝胶过滤色谱法和反相高效液相色谱法对ANP免疫反应性进行分析，结果表明主要免疫反应性与大鼠proANP相对应。卵提取物的半纯化irANP对大鼠主动脉条产生剂量依赖性舒张作用，预先用抗ANP抗血清孵育可阻断该作用。因此，我们认为家蚕卵中合成了ANP。
