[拟南芥和豌豆混合DNA中0.7 kb片段的核苷酸序列分析]
[Analysis of nucleotide sequences of 0.7 kb fragments from mixed DNA from Arabidopsis thaliana and Pisum sativum].
作者信息
Kotliarova E G, Denisenko Iu V, Tarasov V A
出版信息
Genetika. 1994 Apr;30(4):565-9.
HindIII fragments of chloroplast DNA of Pisum sativum L. and Arabidopsis thaliana L. of 0.7-kb size were cloned and sequenced. Comparative analysis of the primary structure of these DNA fragments with that of chloroplast DNA of Nicotianum tabacum L. revealed the presence of a part of the rps19 gene, exon 2 of the rpl2 gene, and the spacer. The sequence homology was evaluated in pairwise comparisons of coding and noncoding sequences, which revealed the specific features of the nucleotide sequences of the studied fragments.
克隆并测序了豌豆（Pisum sativum L.）和拟南芥（Arabidopsis thaliana L.）叶绿体DNA大小为0.7 kb的HindIII片段。将这些DNA片段的一级结构与烟草（Nicotianum tabacum L.）叶绿体DNA的一级结构进行比较分析，发现了rps19基因的一部分、rpl2基因的外显子2和间隔区。通过对编码和非编码序列的成对比较评估序列同源性，揭示了所研究片段核苷酸序列的特定特征。
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