一个与支架相关的DNA区域位于豌豆质体蓝素基因的下游。
A scaffold-associated DNA region is located downstream of the pea plastocyanin gene.
作者信息
Slatter R E, Dupree P, Gray J C
机构信息
Botany School, University of Cambridge, United Kingdom.
出版信息
Plant Cell. 1991 Nov;3(11):1239-50. doi: 10.1105/tpc.3.11.1239.
Abstract
Chromosomal scaffold-associated DNA has been isolated from pea leaf nuclei treated with lithium diiodosalicylate to remove histones and then digested with restriction enzymes to remove the DNA in chromosomal loops. A scaffold-associated region (SAR) of DNA has been identified 8 to 9 kb downstream of the single-copy pea plastocyanin gene in proximity to a repetitive sequence present in 300 copies in the pea haploid genome. Isolated restriction fragments from within the SAR can bind to scaffold preparations in a binding assay in vitro. The nucleotide sequence of the SAR indicates a 540-bp 77% A+T-rich region containing many sequence elements in common with SARs from other organisms. Sequences with homology to topoisomerase II binding sites, A-box and T-box sequences, and replication origins are present within this AT-rich region.
摘要
已从用二碘水杨酸锂处理以去除组蛋白的豌豆叶细胞核中分离出染色体支架相关DNA，然后用限制性内切酶消化以去除染色体环中的DNA。在单拷贝豌豆质体蓝素基因下游8至9 kb处，靠近豌豆单倍体基因组中存在300个拷贝的重复序列，已鉴定出一个DNA支架相关区域（SAR）。在体外结合试验中，从SAR内分离的限制性片段可与支架制剂结合。SAR的核苷酸序列表明有一个540 bp、富含77% A+T的区域，包含许多与其他生物体的SAR共有的序列元件。与拓扑异构酶II结合位点、A盒和T盒序列以及复制起点具有同源性的序列存在于这个富含AT的区域内。
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