Sasaki M, Morita T, Hoso M, Nakanuma Y, Tanimura H
Department of Pathology (II), Kanazawa University School of Medicine, Japan.
Hepatology. 1996 Feb;23(2):258-63. doi: 10.1002/hep.510230210.
We investigated whether carbohydrate antigens on biliary glycoproteins and carcinoembryonic antigen (CEA) are related to hepatolithiasis. CEA, ABO, and Lewis blood group-related antigens, as well as sialyl-Tn antigen in hepatic bile, were analyzed by Western blotting in samples from 12 patients with hepatolithiasis and 37 with other biliary diseases (choledocholithiasis, 13; cholecystolithiasis, 5; acute cholecystitis, 2; cholangiocarcinoma, 5; common bile duct carcinoma, 4; pancreatic carcinoma, 6; and metastatic carcinoma of liver, 2). CEA was positive on mucinous glycoprotein in six patients (50%) with hepatolithiasis and one case (17%) with pancreatic carcinoma. CEA was also positive on a glycoprotein of approximately 200 kd in eight patients (67%) with hepatolithiasis and two (33%) with pancreatic carcinoma. Lewis X was detected on the mucinous glycoprotein in almost all samples, as well as on glycoproteins of approximately 180 kd in all hepatolithiasis samples and approximately half of those from patients with other diseases. Sialyl-Tn antigen was detected on mucinous glycoprotein in four (80%) with cholangiocarcinoma, two (50%) with common bile duct carcinoma, and all pancreatic carcinoma samples. Mucinous glycoprotein and glycoproteins containing CEA and Lewis X antigens are enriched in hepatic bile of hepatolithiasis, and they may be closely related to the formation of intrahepatic calculi. Sialyl-Tn antigen in biliary mucinous glycoprotein will be a good marker of biliary and pancreatic carcinoma, and probably for cholangiocarcinoma complicated with hepatolithiasis.
我们研究了胆汁糖蛋白上的碳水化合物抗原和癌胚抗原（CEA）是否与肝内胆管结石有关。通过蛋白质免疫印迹法分析了12例肝内胆管结石患者和37例其他胆道疾病患者（胆总管结石13例、胆囊结石5例、急性胆囊炎2例、胆管癌5例、胆总管癌4例、胰腺癌6例、肝转移癌2例）肝胆汁中的CEA、ABO和Lewis血型相关抗原以及唾液酸化Tn抗原。CEA在6例（50%）肝内胆管结石患者和1例（17%）胰腺癌患者的黏液糖蛋白上呈阳性。CEA在8例（67%）肝内胆管结石患者和2例（33%）胰腺癌患者中约200kd的糖蛋白上也呈阳性。几乎在所有样本的黏液糖蛋白上均检测到Lewis X，在所有肝内胆管结石样本以及约一半其他疾病患者样本中约180kd的糖蛋白上也检测到Lewis X。在4例（80%）胆管癌、2例（50%）胆总管癌以及所有胰腺癌样本的黏液糖蛋白上检测到唾液酸化Tn抗原。黏液糖蛋白以及含有CEA和Lewis X抗原的糖蛋白在肝内胆管结石患者的肝胆汁中富集，它们可能与肝内结石的形成密切相关。胆道黏液糖蛋白中的唾液酸化Tn抗原将是胆管癌和胰腺癌的良好标志物，可能也是合并肝内胆管结石的胆管癌的良好标志物。
