Kochanek S, Renz D, Doerfler W
Institut für Genetik, Universität Köln, Germany.
Hum Genet. 1994 Aug;94(2):203-6. doi: 10.1007/BF00202872.
In certain segments of human DNA, the methylation of deoxycytidine residues has been found to be highly specific and interindividually conserved. Imprinted DNA sequences in diploid primary cells show allele-specific differences in DNA methylation, usually with the active chromosomal regions being unmethylated and the inactive regions being methylated. We show here that DNA from spermatozoa exhibits variations in allelic methylation patterns. Since germ cells are haploid, individual spermatozoa can differ in DNA methylation patterns not only in the maternally or paternally derived allele, but also within each allele.
在人类DNA的某些片段中，已发现脱氧胞苷残基的甲基化具有高度特异性且个体间保守。二倍体原代细胞中的印记DNA序列在DNA甲基化方面表现出等位基因特异性差异，通常活跃的染色体区域未甲基化，而不活跃区域甲基化。我们在此表明，精子DNA在等位基因甲基化模式上存在差异。由于生殖细胞是单倍体，单个精子在DNA甲基化模式上的差异不仅体现在母源或父源等位基因中，而且在每个等位基因内部也有所不同。
