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YscU是一种参与耶尔森氏菌外膜蛋白（Yop）分泌的小肠结肠炎耶尔森氏菌内膜蛋白。

YscU, a Yersinia enterocolitica inner membrane protein involved in Yop secretion.

作者信息

Allaoui A, Woestyn S, Sluiters C, Cornelis G R

机构信息

Microbial Pathogenesis Unit, Université Catholique de Louvain, Brussels, Belgium.

出版信息

J Bacteriol. 1994 Aug;176(15):4534-42. doi: 10.1128/jb.176.15.4534-4542.1994.

DOI:10.1128/jb.176.15.4534-4542.1994
PMID:8045883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196272/
Abstract

Pathogenic yersiniae secrete antihost Yop proteins by a recently discovered secretion pathway which is also encountered in several animal and plant pathogens. The components of the export machinery are encoded by the virA (lcrA), virB (lcrB), and virC (lcrC) loci of the 70-kb pYV plasmid. In the present paper we describe yscU, the last gene of the virB locus. We determined the DNA sequence and mutated the gene on the pYV plasmid. After inactivation of yscU, the mutant strain was unable to secrete Yop proteins. The topology of YscU was investigated by the analysis of YscU-PhoA translational fusions generated by TnphoA transposition. This showed that the 40.3-kDa yscU product contains four transmembrane segments anchoring a large cytoplasmic carboxyl-terminal domain to the inner membrane. YscU is related to Spa40 from Shigella flexneri, to SpaS from Salmonella typhimurium, to FlhB from Bacillus subtilis, and to HrpN from Pseudomonas solanacearum.

摘要

致病性耶尔森氏菌通过一种最近发现的分泌途径分泌抗宿主Yop蛋白，这种分泌途径在几种动植物病原体中也存在。输出机制的组成部分由70 kb pYV质粒的virA（lcrA）、virB（lcrB）和virC（lcrC）基因座编码。在本文中，我们描述了virB基因座的最后一个基因yscU。我们测定了其DNA序列并在pYV质粒上对该基因进行了突变。yscU失活后，突变菌株无法分泌Yop蛋白。通过分析由TnphoA转座产生的YscU-PhoA翻译融合体来研究YscU的拓扑结构。这表明40.3 kDa的YscU产物包含四个跨膜区段，将一个大的细胞质羧基末端结构域锚定在内膜上。YscU与弗氏志贺氏菌的Spa40、鼠伤寒沙门氏菌的SpaS、枯草芽孢杆菌的FlhB以及青枯假单胞菌的HrpN相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47c3/196272/5cde898a155b/jbacter00033-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47c3/196272/fe92094c9810/jbacter00033-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47c3/196272/5cde898a155b/jbacter00033-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47c3/196272/fe92094c9810/jbacter00033-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47c3/196272/5cde898a155b/jbacter00033-0084-a.jpg

相似文献

1
YscU, a Yersinia enterocolitica inner membrane protein involved in Yop secretion.YscU是一种参与耶尔森氏菌外膜蛋白（Yop）分泌的小肠结肠炎耶尔森氏菌内膜蛋白。
J Bacteriol. 1994 Aug;176(15):4534-42. doi: 10.1128/jb.176.15.4534-4542.1994.
2
Analysis of virC, an operon involved in the secretion of Yop proteins by Yersinia enterocolitica.对耶尔森氏菌小肠结肠炎耶尔森菌分泌Yop蛋白所涉及的操纵子virC的分析。
J Bacteriol. 1991 Aug;173(16):4994-5009. doi: 10.1128/jb.173.16.4994-5009.1991.
3
The lcrB (yscN/U) gene cluster of Yersinia pseudotuberculosis is involved in Yop secretion and shows high homology to the spa gene clusters of Shigella flexneri and Salmonella typhimurium.假结核耶尔森菌的lcrB（yscN/U）基因簇参与耶尔森菌外膜蛋白（Yop）的分泌，并且与痢疾志贺菌和鼠伤寒沙门氏菌的spa基因簇具有高度同源性。
J Bacteriol. 1994 May;176(9):2619-26. doi: 10.1128/jb.176.9.2619-2626.1994.
4
YscN, the putative energizer of the Yersinia Yop secretion machinery.YscN，耶尔森氏菌Yop分泌机制的假定供能体。
J Bacteriol. 1994 Mar;176(6):1561-9. doi: 10.1128/jb.176.6.1561-1569.1994.
5
Proteolytic cleavage of the FlhB homologue YscU of Yersinia pseudotuberculosis is essential for bacterial survival but not for type III secretion.蛋白酶解耶尔森氏假结核耶尔森氏菌的FlhB同源物YscU对细菌存活至关重要，但对III型分泌并非必需。
J Bacteriol. 2002 Aug;184(16):4500-9. doi: 10.1128/JB.184.16.4500-4509.2002.
6
Mutational analysis of the Yersinia enterocolitica virC operon: characterization of yscE, F, G, I, J, K required for Yop secretion and yscH encoding YopR.小肠结肠炎耶尔森菌virC操纵子的突变分析：Yop分泌所需的yscE、F、G、I、J、K以及编码YopR的yscH的特性
Mol Microbiol. 1995 Oct;18(2):343-55. doi: 10.1111/j.1365-2958.1995.mmi_18020343.x.
7
SycE, a chaperone-like protein of Yersinia enterocolitica involved in Ohe secretion of YopE.SycE，一种小肠结肠炎耶尔森菌的伴侣样蛋白，参与YopE的Ohe分泌。
Mol Microbiol. 1993 Apr;8(1):123-31. doi: 10.1111/j.1365-2958.1993.tb01209.x.
8
Role of the transcriptional activator, VirF, and temperature in the expression of the pYV plasmid genes of Yersinia enterocolitica.转录激活因子VirF及温度在小肠结肠炎耶尔森菌pYV质粒基因表达中的作用
Mol Microbiol. 1992 Feb;6(3):395-409.
9
The complete hrp gene cluster of Pseudomonas syringae pv. syringae 61 includes two blocks of genes required for harpinPss secretion that are arranged colinearly with Yersinia ysc homologs.丁香假单胞菌丁香致病变种61的完整hrp基因簇包含两个harpinPss分泌所需的基因块，它们与耶尔森氏菌ysc同源物呈共线性排列。
Mol Plant Microbe Interact. 1995 Sep-Oct;8(5):733-46. doi: 10.1094/mpmi-8-0733.
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Erwinia amylovora secretes harpin via a type III pathway and contains a homolog of yopN of Yersinia spp.梨火疫病菌通过III型分泌途径分泌harpin蛋白，并且含有耶尔森氏菌属yopN的同源物。
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Bacterial type III secretion systems: a complex device for the delivery of bacterial effector proteins into eukaryotic host cells.细菌III型分泌系统：一种将细菌效应蛋白递送至真核宿主细胞的复杂装置。

本文引用的文献

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Multiple effects of lcrD mutations in Yersinia pestis.鼠疫耶尔森氏菌中lcrD突变的多种效应。
J Bacteriol. 1993 Jun;175(11):3536-45. doi: 10.1128/jb.175.11.3536-3545.1993.
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SycE, a chaperone-like protein of Yersinia enterocolitica involved in Ohe secretion of YopE.SycE，一种小肠结肠炎耶尔森菌的伴侣样蛋白，参与YopE的Ohe分泌。
Mol Microbiol. 1993 Apr;8(1):123-31. doi: 10.1111/j.1365-2958.1993.tb01209.x.
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Membrane traffic wardens and protein secretion in gram-negative bacteria.革兰氏阴性菌中的膜运输调控蛋白与蛋白质分泌
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The Third Transmembrane Domain of EscR Is Critical for Function of the Enteropathogenic Type III Secretion System.EscR 的第三个跨膜结构域对肠致病性 III 型分泌系统的功能至关重要。
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HpaB-Dependent Secretion of Type III Effectors in the Plant Pathogens Ralstonia solanacearum and Xanthomonas campestris pv. vesicatoria.HpaB 依赖型 III 型效应物分泌在植物病原菌罗尔斯顿氏菌和野油菜黄单胞菌 pv.vesicatoria 中的作用。
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The Ruler Protein EscP of the Enteropathogenic Escherichia coli Type III Secretion System Is Involved in Calcium Sensing and Secretion Hierarchy Regulation by Interacting with the Gatekeeper Protein SepL.肠致病性大肠杆菌III型分泌系统的尺子蛋白EscP通过与守门蛋白SepL相互作用参与钙感知和分泌层级调节。
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BMC Microbiol. 2016 May 20;16:88. doi: 10.1186/s12866-016-0702-y.
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YscU/FlhB of Yersinia pseudotuberculosis Harbors a C-terminal Type III Secretion Signal.假结核耶尔森菌的YscU/FlhB含有一个C端III型分泌信号。
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Cognate gene clusters govern invasion of host epithelial cells by Salmonella typhimurium and Shigella flexneri.同源基因簇控制鼠伤寒沙门氏菌和福氏志贺氏菌对宿主上皮细胞的侵袭。
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Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a.位于5区形成操纵子的8个基因对福氏志贺菌2a侵袭上皮细胞至关重要。
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Regulation by Ca2+ in the Yersinia low-Ca2+ response.耶尔森氏菌低钙应答中钙离子的调控作用
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Pseudomonas syringae pv. syringae harpinPss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants.丁香假单胞菌丁香致病变种的harpinPss蛋白：一种通过Hrp途径分泌并在植物中引发过敏反应的蛋白质。
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Homology between the HrpO protein of Pseudomonas solanacearum and bacterial proteins implicated in a signal peptide-independent secretion mechanism.青枯雷尔氏菌的HrpO蛋白与涉及不依赖信号肽分泌机制的细菌蛋白之间的同源性。
Mol Gen Genet. 1993 Jun;239(3):378-92. doi: 10.1007/BF00276936.
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PopA1, a protein which induces a hypersensitivity-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum.PopA1是一种能在特定矮牵牛基因型上引发类似过敏反应的蛋白质，它通过青枯雷尔氏菌的Hrp途径分泌。
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