Tiran A, Pieber T, Tiran B, Halwachs-Baumann G, Dobnig H, Grubelnig H, Wilders-Truschnig M M
Medizinische Universitätsklinik, Karl-Franzens Universität, Graz, Austria.
J Clin Lab Anal. 1994;8(3):128-34. doi: 10.1002/jcla.1860080303.
We evaluated four newly introduced assays for determination of glycated hemoglobin allowing the processing of large amounts of samples in a clinical routine laboratory. These methods were compared to the Bio-Rad Diamat system. The investigated methods were the Merck Hitachi L-9100, a fully automated HPLC analyser, the Abbott IMx glycated hemoglobin ion capture assay, the DAKO HbA1c enzyme linked immunosorbent assay (ELISA), and the Boehringer-Mannheim HbA1c Tinaquant turbidimetric assay. All methods showed generally acceptable precision and good accordance with the Diamat system. Interference study showed influence of anaemia, polycythemia, rheumatoid factor, and chronic hemodialysis on the values of the DAKO ELISA and of anaemia and polycythemia on the values of the Boehringer-Mannheim Tinaquant assay. All of the investigated methods allow referring of results either as measured or standardized HbA1c values, the latter obtained after calibration with reference to an ion exchange high-pressure liquid chromatography method. Our data confirm the feasibility of this kind of standardisation of glycated hemoglobin assays, allowing direct comparison of results from various determination methods.
我们评估了四种新引入的糖化血红蛋白检测方法，这些方法可在临床常规实验室中处理大量样本。将这些方法与Bio-Rad Diamat系统进行了比较。所研究的方法包括默克日立L-9100全自动高效液相色谱分析仪、雅培IMx糖化血红蛋白离子捕获检测法、DAKO HbA1c酶联免疫吸附测定法（ELISA）以及勃林格殷格翰HbA1c Tinaquant比浊法。所有方法的精密度总体上均可接受，且与Diamat系统具有良好的一致性。干扰研究表明，贫血、红细胞增多症、类风湿因子和慢性血液透析会影响DAKO ELISA法的检测值，而贫血和红细胞增多症会影响勃林格殷格翰Tinaquant法的检测值。所有研究方法均可将结果表示为实测糖化血红蛋白值或标准化糖化血红蛋白值，后者是参照离子交换高压液相色谱法校准后获得的。我们的数据证实了这种糖化血红蛋白检测标准化的可行性，使得不同检测方法的结果能够直接比较。
