Tsuda T, Kita S
Third Department of Medicine, Oita Medical University.
Nihon Rinsho. 1994 Jun;52(6):1456-61.
Immunohistochemical study of sarcoid granulomatous lesions. In first we studied the distribution of each functionally distinct T lymphocyte subsets in sarcoid lesions by use of a single and double stain methods with monoclonal antibodies for T cell markers including CD4, CD8, CD45RA, and CD45RO. The results showed that CD4(+)+CD45RA- T cells "Helper/helper inducer" distributed diffusely though the lesions and CD8(+)+CD45RA- T cells "Cytotoxic" distributed at edge of the granulomas. And CD4(+)+CD45RA+ T cells "Suppressor inducer" and CD8(+)+CD45RA+ T cells "Suppressor" also distributed near the granulomas. In second we studied populations of T cells expressed with each antigen including CD26, 28, 29, 11a, 54, 43, 58, 69, 45RA, 45RO, and CD25. The results showed that T cells expressed with each antigen as described above were more numerously in granulomas than out of granulomas. In third we studied T cells designated as T helper 1 (Th-1) and T helper 2 (Th-2) by use of ant-IFN gamma for Th-1 and anti-IL4 and IL6 for Th-2. The results showed that IFN gamma producing cells were scantily demonstrated out of granulomas and conversely IL4 and IL6 producing cells were demonstrated more numerously in granulomas than out of granulomas. From above results we concluded that Immunologic event in sarcoidosis might be Th-2 biased response.
结节病肉芽肿性病变的免疫组织化学研究。首先，我们通过使用针对包括CD4、CD8、CD45RA和CD45RO在内的T细胞标志物的单克隆抗体的单染和双染方法，研究了结节病病变中每个功能不同的T淋巴细胞亚群的分布。结果显示，CD4(+)+CD45RA-T细胞“辅助/辅助诱导细胞”在病变中弥漫分布，而CD8(+)+CD45RA-T细胞“细胞毒性细胞”分布在肉芽肿边缘。并且CD4(+)+CD45RA+T细胞“抑制诱导细胞”和CD8(+)+CD45RA+T细胞“抑制细胞”也分布在肉芽肿附近。其次，我们研究了表达每种抗原的T细胞群体，这些抗原包括CD26、28、29、11a、54、43、58、69、45RA、45RO和CD25。结果显示，表达上述每种抗原的T细胞在肉芽肿中比在肉芽肿外数量更多。第三，我们通过使用抗IFNγ检测Th-1和抗IL4及IL6检测Th-2来研究被指定为Th1和Th2的T细胞。结果显示，产生IFNγ的细胞在肉芽肿外很少见，相反，产生IL4和IL6的细胞在肉芽肿中比在肉芽肿外数量更多。根据上述结果，我们得出结论，结节病中的免疫事件可能是Th2偏向性反应。
