Kita S, Tsuda T, Sugisaki K, Miyazaki E, Matsumoto T
Third Department of Internal Medicine, Oita Medical University.
Intern Med. 1995 Sep;34(9):847-55. doi: 10.2169/internalmedicine.34.847.
We studied the relationship between various T lymphocyte functions and granuloma formation in 5 lung tissue and 4 lymph node tissue samples from patients with sarcoidosis by immunohistological methods. In the lesion of sarcoidosis, T cells were positive for alpha beta TCR, but gamma delta TCR-positive T cells were rarely observed. The results of analysis of functional subsets showed that T cells in the internal area of granuloma were predominantly helper/inducer subset (CD4+CD45RA-). On the other hand, cytotoxic T cells (CD8+CD45RA-CD11b-) were present in abundance in the outer boundaries of granuloma. In addition, suppressor-inducer T cells (CD4+CD45RA+) were present in the surrounding areas. However, T cells of various subsets were present sporadically outside the granulomas. We also studied the distribution of T cells expressing activation-related antigens. The results showed that T lymphocytes in the internal area of granulomas more frequently had these antigens than did T lymphocytes in the external area. These findings suggested that T cells infiltrating into the sarcoidosis lesion demonstrated a layer-like distribution based on functional subsets. These findings also confirm that activated T cells were more abundantly distributed in the internal area of sarcoid granuloma than in the external area.
我们采用免疫组织学方法，对5例结节病患者的肺组织样本和4例淋巴结组织样本中各种T淋巴细胞功能与肉芽肿形成之间的关系进行了研究。在结节病病变中，T细胞αβTCR呈阳性，但很少观察到γδTCR阳性T细胞。功能亚群分析结果显示，肉芽肿内部区域的T细胞主要为辅助/诱导亚群（CD4+CD45RA-）。另一方面，细胞毒性T细胞（CD8+CD45RA-CD11b-）大量存在于肉芽肿的外边界。此外，抑制诱导T细胞（CD4+CD45RA+）存在于周围区域。然而，各种亚群的T细胞在肉芽肿外呈散在分布。我们还研究了表达激活相关抗原的T细胞的分布情况。结果显示，肉芽肿内部区域的T淋巴细胞比外部区域的T淋巴细胞更频繁地表达这些抗原。这些发现表明，浸润到结节病病变中的T细胞基于功能亚群呈层状分布。这些发现还证实，活化的T细胞在结节病肉芽肿内部区域的分布比外部区域更为丰富。
