台湾地区人类沙门氏菌分离株中Qnr决定簇的出现。
Emergence of Qnr determinants in human Salmonella isolates in Taiwan.
作者信息
Wu Jiunn-Jong, Ko Wen-Chien, Chiou Chien-Shun, Chen Hung-Mo, Wang Li-Ron, Yan Jing-Jou
机构信息
Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University Medical College, Tainan 70101, Taiwan.
出版信息
J Antimicrob Chemother. 2008 Dec;62(6):1269-72. doi: 10.1093/jac/dkn426. Epub 2008 Oct 27.
OBJECTIVES
The aim of this study was to determine the prevalence and characteristics of qnr-carrying Salmonella isolates from humans in southern Taiwan.
METHODS
A total of 446 Salmonella isolates collected between 2003 and 2006 were screened for qnrA, qnrB and qnrS by PCR experiments. Genetic structures of qnr were determined by PCR-based methods or direct sequencing of plasmid DNA.
RESULTS
qnrB2 and qnrS1 were detected in two serovar Enteritidis isolates and two serovar Typhimurium isolates, respectively. One qnrS1-positive isolate was found to produce the CMY-2 AmpC enzyme. qnrS1 was identified on a 10 kb plasmid, which exhibited >99% nucleotide sequence identity with plasmid TPqnrS-1a reported from the UK. qnrB2 was found in a complex sul1-type class 1 integron on a >100 kb plasmid.
CONCLUSIONS
This study demonstrated the occurrence of qnrB2 and qnrS1 in Salmonella for the first time in Taiwan and characterized their genetic structures.
目的
本研究旨在确定台湾南部人类沙门氏菌分离株中携带qnr基因的流行情况及特征。
方法
通过PCR实验对2003年至2006年间收集的446株沙门氏菌分离株进行qnrA、qnrB和qnrS基因筛查。采用基于PCR的方法或质粒DNA直接测序确定qnr基因的遗传结构。
结果
在两株肠炎沙门氏菌分离株和两株鼠伤寒沙门氏菌分离株中分别检测到qnrB2和qnrS1。发现一株qnrS1阳性分离株可产生CMY-2 AmpC酶。qnrS1定位于一个10 kb的质粒上，该质粒与英国报道的质粒TPqnrS-1a的核苷酸序列同一性>99%。qnrB2存在于一个大于100 kb质粒上的复杂sul1型1类整合子中。
结论
本研究首次在台湾的沙门氏菌中证实了qnrB2和qnrS1的存在，并对其遗传结构进行了表征。
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