de Bruijn W C, van Mourik W
Virchows Arch A Pathol Anat Histol. 1975;365(1):23-40. doi: 10.1007/BF00439283.
Following the administration of cholesterol for a period of 6-7 weeks, Scanning Electron Microscopic (S.E.M.) observations revealed mono-cellular, crater-like and dome-shaped endothelial changes on top of the large intimal plaques in the rabbit aortas. Finger-like and other shaped cell protrusions were observed at the edges of these crater-like and dome-shaped endothelial changes, giving the intimal plaques a rough appearance. At other sites, normal, smooth, although irregularly arranged, endothelial cells covered the lesions. By impregnating the cell borders with silver-nitrate or silver proteinate containing perfusates, it was possible in most cases to ascertain that the lesions were derived from changes in one cell or from changes in a small collection of cells. S.E.M.-observations further revealed crater-like and dome-shaped endothelial changes to be present in large fields or as isolated cell changes in normal areas at sites where no gross lesions were observed with the light microscope. In addition large, multi-cellular, crate-like endothelial changes were observed at the edges of the large intimal plaques. At these sites several endothelial cells were lacking, leaving behind a crater in which sometimes cells and a few fibrin threads were found. Following the administration of cholesterol for periods of 4-5 and 2-3 weeks similar monocellular changes were observed, some extending over large areas, other as single cells in apparently normal surroundings. Quantitatively the number of lesions was less than when the cholesterol was administered for a longer period. Transmission electron microscopic studies revealed the presence of large amounts of membrane-bound lipid globules in the subendothelial spaces and within some endothelial cells, structures which were assumed to be cross-sections of the crater-like or dome-shaped endothelial cell protrusions, visible with the S.E.M.
在给予胆固醇6 - 7周后，扫描电子显微镜（S.E.M.）观察显示，兔主动脉大内膜斑块顶部出现单细胞、火山口状和圆顶状内皮改变。在这些火山口状和圆顶状内皮改变的边缘观察到指状和其他形状的细胞突起，使内膜斑块外观粗糙。在其他部位，正常、光滑但排列不规则的内皮细胞覆盖着病变。通过用含硝酸银或蛋白银的灌注液浸染细胞边界，在大多数情况下可以确定病变源自一个细胞的变化或一小群细胞的变化。S.E.M.观察进一步显示，在光镜下未观察到明显病变的部位，火山口状和圆顶状内皮改变存在于大片区域或作为正常区域的孤立细胞改变。此外，在大内膜斑块的边缘观察到大型、多细胞、板条样内皮改变。在这些部位，几个内皮细胞缺失，留下一个火山口，有时在其中发现细胞和一些纤维蛋白丝。在给予胆固醇4 - 5周和2 - 3周后，观察到类似的单细胞改变，一些改变延伸至大片区域，另一些作为明显正常环境中的单个细胞。从数量上看，病变数量比给予胆固醇更长时间时少。透射电子显微镜研究显示，在内皮下间隙和一些内皮细胞内存在大量膜结合脂质球，这些结构被认为是S.E.M.可见的火山口状或圆顶状内皮细胞突起的横截面。
