Schimmer O, Krüger A, Paulini H, Haefele F
Institut für Botanik und Pharmazeutische Biologie, Universität Erlangen-Nürnberg.
Pharmazie. 1994 Jun;49(6):448-51.
55 commercial phytopharmaceuticals (extracts and tinctures) from 44 plant species were evaluated for mutagenic potential in the Salmonella/mammalian microsome mutagenicity test (Ames assay), utilizing tester strains TA98 and TA100 of Salmonella typhimurium with and without S9 mix from induced rat liver microsomes. Weak activities were detected after exposure of the bacteria to Alchemillae tinctura, Centaurii extractum, Hippocastani extractum and Myrtilli extractum. Moderate effects were observed with Crataegi extractum, Echinaceae angustifoliae extractum, Hyperici tinctura, Rutae tinctura and Trifolii fibrini extractum and tinctura. Quercetin was detected by TLC in all extracts with mutagenic activity except in Echinaceae angustifoliae and Centaurii extractum. From this study and earlier results we suggest that quercetin is possibly the main mutagenic principle in the following phytopharmaceuticals: Alchemillae tinctura, Cratagei extractum, Hippocastani extractum, Hyperici tinctura, Myrtilli extractum, Trifolii fibrini extractum and Trifolii fibrini tinctura.
利用鼠伤寒沙门氏菌TA98和TA100测试菌株，在有或没有来自诱导大鼠肝微粒体的S9混合物的情况下，对来自44种植物的55种商业植物药（提取物和酊剂）进行了沙门氏菌/哺乳动物微粒体诱变性试验（艾姆斯试验）中的诱变潜力评估。将细菌暴露于地榆酊、矢车菊提取物、七叶树提取物和蓝莓提取物后，检测到微弱活性。在山楂提取物、狭叶紫锥菊提取物、金丝桃酊、芸香酊和红车轴草提取物及酊剂中观察到中等效果。除狭叶紫锥菊和矢车菊提取物外，在所有具有诱变活性的提取物中通过薄层色谱法检测到了槲皮素。根据本研究及早期结果，我们认为槲皮素可能是以下植物药中的主要诱变成分：地榆酊、山楂提取物、七叶树提取物、金丝桃酊、蓝莓提取物、红车轴草提取物和红车轴草酊。
