Nordström K, Wagner E G
Department of Microbiology, Uppsala University, Sweden.
Trends Biochem Sci. 1994 Jul;19(7):294-300. doi: 10.1016/0968-0004(94)90008-6.
Plasmids are replicating DNA molecules that are present in defined numbers of copies per cell. They encode systems that control their replication such that given steady-state values for their copy numbers are maintained. This is a special type of control, since it requires the genome to measure its concentration continuously and adjust its rate of replication to parallel the rate of growth of the cell mass. In this review we discuss the quantitative kinetic properties of copy-number control of the R1 plasmid, in which the control device is an antisense RNA that controls the synthesis of a protein that is rate-limiting for replication of the plasmid.
质粒是可复制的DNA分子，每个细胞中质粒的拷贝数是确定的。它们编码控制自身复制的系统，从而维持特定的稳态拷贝数。这是一种特殊类型的控制，因为它要求基因组持续测量其浓度，并调整其复制速率以与细胞质量的生长速率保持一致。在本综述中，我们讨论了R1质粒拷贝数控制的定量动力学特性，其中控制装置是一种反义RNA，它控制一种对质粒复制起限速作用的蛋白质的合成。
