Edwards T, McBride B C
Appl Microbiol. 1975 Apr;29(4):540-5. doi: 10.1128/am.29.4.540-545.1975.
A new technique is reported for the rapid growth and detection of methanogenic bacteria by using petri plates. The method employs an anaerobic glove box containing an inner chamber with separate gas-flushing facilities. The numbers of methanogenic bacteria recovered from domestic sewage sludge are comparable to those recovered by other methods. The methanogenic organisms isolated from sludge include Methanosarcina, Methanospirillum, Methanobacterium strain M.o.H., and Methanobacterium formicicum. Identification of colonies containing methanogenic bacteria is facilitated by taking advantage of the unique fluorescence properties of these organisms. Colonies as small as 0.5 mm can be detected by exposing them to long-wave ultraviolet light.
据报道，一种利用培养皿快速培养和检测产甲烷菌的新技术。该方法采用一个厌氧手套箱，其内部腔室设有独立的气体冲洗设施。从生活污水污泥中回收的产甲烷菌数量与通过其他方法回收的数量相当。从污泥中分离出的产甲烷微生物包括甲烷八叠球菌、甲烷螺菌、M.o.H.甲烷杆菌菌株和甲酸甲烷杆菌。利用这些微生物独特的荧光特性有助于鉴定含有产甲烷菌的菌落。通过将菌落暴露在长波紫外线下，可以检测到小至0.5毫米的菌落。
