Li Shujiang, Fang Xinmei, Zhang Hanlian, Zeng Yanling, Zhu Tianhui
College of Forestry, Sichuan Agricultural University, Chengdu 611130, Sichuan Province, China.
Plant Pathol J. 2019 Jun;35(3):234-242. doi: 10.5423/PPJ.OA.09.2018.0187. Epub 2019 Jun 1.
Thirty-four strains of bacteria were isolated from . Using as an indicator strain, the best strain, B18, was obtained by the growth rate method. The morphological, physiological and biochemical characteristics of strain B18 and its 16S DNA gene sequence were identified, and the biocontrol effect of strain B18 was assessed in pot and field tests, as well as in a field-control test. Drilling methods were used to determine the antibacterial activity of metabolites from strain B18 and their effects on the growth of pathogen mycelia and spores. The best bacteriostatic rate was 85.4%. B18 can hydrolyse starch and oxidize glucose but does not produce gas; a positive result was obtained in a gelatine liquefaction test. According to 16S DNA gene sequencing, strain B18 is (GenBank accession number: MG457759). The results of pot and field-control trials showed 98% disease control when inoculating 10 cfu/ml of the strain. The disease control effect of the B18 culture liquid (concentrations of 10, 2 × 10, 10, 5 × 10 and 2.5 × 10 cfu/ml) in the field-control test was higher than 80%, and the cure rate of the original delivery solution was 96%. Therefore, in the practical forestry production, a 2.5 × 10 cfu/ml culture liquidshould be applied in advance to achieve good control effects.
从……分离出34株细菌。以……作为指示菌株，通过生长速率法获得了最佳菌株B18。对菌株B18的形态、生理生化特性及其16S DNA基因序列进行了鉴定，并在盆栽和田间试验以及田间对照试验中评估了菌株B18的生防效果。采用打孔法测定了菌株B18代谢产物的抑菌活性及其对病原菌菌丝体和孢子生长的影响。最佳抑菌率为85.4%。B18能水解淀粉并氧化葡萄糖，但不产气；明胶液化试验结果为阳性。根据16S DNA基因测序，菌株B18为……（GenBank登录号：MG457759）。盆栽和田间对照试验结果表明，接种10 cfu/ml该菌株时病害防治率为98%。田间对照试验中B18培养液（浓度为10、2×10、10、5×10和2.5×10 cfu/ml）的病害防治效果高于80%，原液治愈率为96%。因此，在实际林业生产中，应提前施用2.5×10 cfu/ml的培养液以取得良好的防治效果。
