果蝇和L细胞DNA中多嘧啶的特征分析。

Characterization of polypyrimidines in Drosophila and L-cell DNA.

作者信息

Birnboim H C, Straus N A, Sederoff R R

出版信息

Biochemistry. 1975 Apr 22;14(8):1643-7. doi: 10.1021/bi00679a016.

Abstract

Unusually long pyrimidine tracts (polypyrimidines), ranging from 100 to over 1000 nucleotides in length, have been found in Drosophila melanogaster DNA. They are compared to shorter pyrimidine tracts (25-150 nucleotides) which have previously been found in L-cell DNA. Both species were able to anneal to homologous DNA; Drosophila polypyrimidines formed stable hybrids while L-cell polypyrimidines formed hybrids of lower thermal stability. In both cases, the kinetics of the reaction was rapid, suggesting that these tracts are part of highly repeated DNA.

摘要

在黑腹果蝇的DNA中发现了异常长的嘧啶序列（多嘧啶序列），长度从100到1000多个核苷酸不等。将它们与之前在L细胞DNA中发现的较短嘧啶序列（25 - 150个核苷酸）进行比较。两种序列都能够与同源DNA退火；果蝇的多嘧啶序列形成稳定的杂交体，而L细胞的多嘧啶序列形成热稳定性较低的杂交体。在这两种情况下，反应动力学都很快，这表明这些序列是高度重复DNA的一部分。