Mu J H, Stains J P, Kao T
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802.
Plant Mol Biol. 1994 Jun;25(3):539-44. doi: 10.1007/BF00043881.
From a pollen tube cDNA library of Petunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing the PPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in the PPE1 gene. During pollen development, PPE1 mRNA was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level in in vitro germinated pollen tubes. The observed expression pattern of the PPE1 gene suggests that its product may play a role in pollen germination and/or tube growth.
从矮牵牛的花粉管cDNA文库中，我们分离出了编码一种名为PPE1蛋白质的cDNA克隆，该蛋白质与植物、细菌和真菌的果胶酯酶具有序列相似性。以PPE1的cDNA为探针，分离出了包含PPE1基因的基因组克隆，对cDNA和基因组序列的比较显示PPE1基因中存在一个内含子。在花粉发育过程中，PPE1 mRNA首先在含有单核小孢子的花药中被检测到；在成熟花粉中达到最高水平，并在体外萌发的花粉管中持续高水平存在。PPE1基因观察到的表达模式表明其产物可能在花粉萌发和/或花粉管生长中起作用。
