Marks S L, Dupuis J, Mickelsen W D, Memon M A, Platz C C
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman 99164.
J Am Vet Med Assoc. 1994 May 15;204(10):1639-40.
A 9-year-old sexually intact male Boxer was euthanatized because of progressive, generalized seizures that were refractory to medical treatment. To try to preserve the breeding line, postmortem epididymal semen extraction was attempted. The testes were excised after the dog was euthanatized, and semen was extracted, frozen, and stored in liquid nitrogen. Approximately 3.5 months later, a suitable recipient was identified, and surgical intrauterine insemination was performed. Fifty-seven days after insemination, a viable pup was born.
一只9岁未绝育的雄性拳师犬因进行性全身性癫痫发作且药物治疗无效而被安乐死。为了保留该繁殖系,尝试进行死后附睾精液采集。犬被安乐死后切除睾丸,采集精液,冷冻并储存于液氮中。大约3.5个月后,确定了合适的受体,并进行了手术子宫内授精。授精57天后,一只存活的幼犬出生。