Gouvea V, Santos N, Timenetsky M do C
Division of Molecular Biological Research and Evaluation, Food and Drug Administration, Washington, D.C. 20204.
J Clin Microbiol. 1994 May;32(5):1333-7. doi: 10.1128/jcm.32.5.1333-1337.1994.
A PCR typing assay was developed to identify rotavirus P types (VP4 specificity) of bovine NCDV, UK, and B223 strains and porcine OSU and Gottfried strains. Thirty-nine human and animal strains representing all known, and some undefined, rotavirus P types were used to develop and evaluate the specificity of the method. No cross-amplification was observed. The PCR results agreed with previous characterizations by monoclonal antibodies, sequence analysis, and hybridization assays, except for the Gottfried strain, which showed a P type distinct from the human asymptomatic strains. Analysis of a small number of field specimens suggested a high level of VP4 polymorphism among porcine strains. The assay should be of value in typing field isolates and tracing interspecies infections.
开发了一种聚合酶链反应（PCR）分型检测方法，用于鉴定牛NCDV、英国株和B223株以及猪OSU株和戈特弗里德株的轮状病毒P型（VP4特异性）。使用代表所有已知和一些未定义轮状病毒P型的39株人和动物毒株来开发和评估该方法的特异性。未观察到交叉扩增。除戈特弗里德株外，PCR结果与先前通过单克隆抗体、序列分析和杂交检测的分型结果一致，戈特弗里德株显示出与人类无症状毒株不同的P型。对少量现场样本的分析表明，猪毒株中VP4多态性水平较高。该检测方法在对现场分离株进行分型和追踪种间感染方面应具有价值。
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