Kenny M T, Brackman M A
Marion Merrell Dow, Inc., Kansas City, Missouri 64134-0627.
J Clin Microbiol. 1994 May;32(5):1364-5. doi: 10.1128/jcm.32.5.1364-1365.1994.
Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube dilution susceptibility tests gave essentially the same results (93.9% of the test results were within 1 doubling dilution of equivalence), (ii) broth microdilution susceptibility tests gave results up to 5 doubling dilutions higher than agar or tube assays, and (iii) this data skewing could be reversed by BSA supplementation (final concentration, 0.02%) of the broth microdilution test medium.
比较了标准肉汤微量稀释法(添加和不添加牛血清白蛋白[BSA])、试管稀释法和琼脂稀释药敏试验以确定雷莫拉宁的最低抑菌浓度(MIC)。以来自美国33个地点的246株革兰氏阳性菌临床分离株为数据库,结果表明:(i)琼脂稀释和试管稀释药敏试验结果基本相同(93.9%的试验结果在等效浓度的1个稀释倍数范围内);(ii)肉汤微量稀释药敏试验结果比琼脂或试管试验结果高5个稀释倍数;(iii)在肉汤微量稀释试验培养基中添加BSA(终浓度为0.02%)可逆转这种数据偏差。