用于测定雷莫拉宁最低抑菌浓度（MIC）的琼脂稀释法、试管稀释法和肉汤微量稀释法药敏试验的比较。
Comparison of agar dilution, tube dilution, and broth microdilution susceptibility tests for determination of ramoplanin MICs.
作者信息
Kenny M T, Brackman M A
机构信息
Marion Merrell Dow, Inc., Kansas City, Missouri 64134-0627.
出版信息
J Clin Microbiol. 1994 May;32(5):1364-5. doi: 10.1128/jcm.32.5.1364-1365.1994.
Abstract
Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube dilution susceptibility tests gave essentially the same results (93.9% of the test results were within 1 doubling dilution of equivalence), (ii) broth microdilution susceptibility tests gave results up to 5 doubling dilutions higher than agar or tube assays, and (iii) this data skewing could be reversed by BSA supplementation (final concentration, 0.02%) of the broth microdilution test medium.
摘要
比较了标准肉汤微量稀释法（添加和不添加牛血清白蛋白[BSA]）、试管稀释法和琼脂稀释药敏试验以确定雷莫拉宁的最低抑菌浓度（MIC）。以来自美国33个地点的246株革兰氏阳性菌临床分离株为数据库，结果表明：（i）琼脂稀释和试管稀释药敏试验结果基本相同（93.9%的试验结果在等效浓度的1个稀释倍数范围内）；（ii）肉汤微量稀释药敏试验结果比琼脂或试管试验结果高5个稀释倍数；（iii）在肉汤微量稀释试验培养基中添加BSA（终浓度为0.02%）可逆转这种数据偏差。
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本文引用的文献
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