Sundsfjord A, Spein A R, Lucht E, Flaegstad T, Seternes O M, Traavik T
Department of Virology, School of Medicine, University of Tromsø, Norway.
J Clin Microbiol. 1994 May;32(5):1390-4. doi: 10.1128/jcm.32.5.1390-1394.1994.
Our understanding of important stages in the pathogenesis of the human polyomavirus BK virus (BKV) and JC virus (JCV) infections is limited. In this context, nasopharyngeal aspirates from 201 children with respiratory diseases and saliva from 60 human immunodeficiency virus type 1-infected adults and 10 healthy adult controls were collected and analyzed for the presence of BKV and JCV DNA by PCR. Neither BKV nor JCV DNA was detected in the saliva specimens. We demonstrated BKV DNA, but no infectious BKV, in 2 of 201 nasopharyngeal aspirates. Each sample contained one unique rearranged noncoding control region variant of BKV. The results indicate that (i) BKV and JCV are not regularly associated with respiratory infections in children requiring hospitalization, (ii) nasopharyngeal cells are not an important site for primary replication of human polyomavirus BKV and JCV, and (iii) the salivary glands and oropharyngeal cells seem not to be involved in BKV and JCV persistence. We propose that for the polyomaviruses BKV and JCV the alimentary tract should be considered as a portal of entrance to the human organism.
我们对人类多瘤病毒BK病毒（BKV）和JC病毒（JCV）感染发病机制中重要阶段的了解有限。在此背景下，收集了201名患有呼吸道疾病儿童的鼻咽抽吸物、60名感染1型人类免疫缺陷病毒的成人以及10名健康成人对照的唾液，并通过聚合酶链反应（PCR）分析其中BKV和JCV DNA的存在情况。在唾液标本中未检测到BKV和JCV DNA。我们在201份鼻咽抽吸物中的2份中检测到了BKV DNA，但未检测到具有感染性的BKV。每个样本都包含一个独特的BKV重排非编码控制区变体。结果表明：（i）BKV和JCV与需要住院治疗的儿童呼吸道感染无常规关联；（ii）鼻咽细胞不是人类多瘤病毒BKV和JCV主要复制的重要部位；（iii）唾液腺和口咽细胞似乎不参与BKV和JCV的持续存在。我们提出，对于多瘤病毒BKV和JCV，应将消化道视为进入人体的门户。
