Lucht E, Albert J, Linde A, Xu W, Brytting M, Lundeberg J, Uhlén M, Bratt G, Sandström E, Heimdahl A
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
J Med Virol. 1993 Feb;39(2):156-62. doi: 10.1002/jmv.1890390213.
The aim of this study was to evaluate whether HIV-1 or cytomegalovirus (CMV) may contribute to oral lesions frequently found in patients with the acquired immunodeficiency syndrome (AIDS). Saliva samples from 63 HIV-1 positive patients and 21 healthy controls were tested for the presence of HIV-1 and CMV using the polymerase chain reaction (PCR) and virus isolation. CMV IgG titres in serum were also compared in the different groups. HIV-1 RNA, but not DNA, was detected in saliva from 15% (9 out of 59) of the HIV-infected patients. There was no correlation between the presence of HIV-1 RNA and oral symptoms in the patients. CMV DNA was detected significantly more frequently in samples from HIV-1 seropositive than from seronegative patients. CMV was detected in saliva from AIDS patients more often than in saliva from patients with mild or no symptoms. CMV IgG titres were also significantly higher in symptomatic than in asymptomatic patients. There was a significant correlation between the presence of CMV DNA and necrotizing gingivitis, and oral Kaposi's sarcoma in the patients, and also between these lesions and the stage of disease. This does not prove that CMV causes these oral lesions, but a direct or indirect role for CMV cannot be excluded.
本研究的目的是评估人类免疫缺陷病毒1型（HIV-1）或巨细胞病毒（CMV）是否可能导致获得性免疫缺陷综合征（AIDS）患者中常见的口腔病变。使用聚合酶链反应（PCR）和病毒分离技术，对63例HIV-1阳性患者和21例健康对照的唾液样本进行HIV-1和CMV检测。同时比较不同组血清中CMV IgG滴度。在15%（59例中的9例）HIV感染患者的唾液中检测到HIV-1 RNA，但未检测到DNA。患者中HIV-1 RNA的存在与口腔症状之间无相关性。与血清阴性患者相比，HIV-1血清阳性患者样本中CMV DNA的检测频率显著更高。AIDS患者唾液中CMV的检测频率高于症状轻微或无症状患者的唾液。有症状患者的CMV IgG滴度也显著高于无症状患者。患者中CMV DNA的存在与坏死性牙龈炎和口腔卡波西肉瘤之间存在显著相关性，这些病变与疾病阶段之间也存在显著相关性。这并不能证明CMV会导致这些口腔病变，但不能排除CMV的直接或间接作用。
