Zipeto D, Sarasini A, Rossi F, Baldanti F, Revello M G, Milanesi G, Gerna G
Instituto di Genetica Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Pavia, Italy.
J Clin Microbiol. 1994 May;32(5):1402-5. doi: 10.1128/jcm.32.5.1402-1405.1994.
In an AIDS patient with a disseminated human cytomegalovirus (HCMV) infection, presence of HCMV in blood was repeatedly excluded by the shell vial culture method with the HCMV immediate-early (IE) antigen-specific monoclonal antibody (MAb) 5D2 currently employed for rapid HCMV identification, whereas it was repeatedly confirmed by all other assays (conventional virus isolation from blood, antigenemia, and DNAemia). Sequence analysis of the HCMV strain revealed a point mutation in exon 2 of the IE gene, which led to a serine-to-proline substitution at position 20 of the corresponding protein. Cloning and expression of a region of the IE gene containing the mutation showed that this was responsible for the lack of reactivity of MAb 5D2. A pool of IE antigen-reactive MAbs instead of a single MAb must be used for rapid HCMV identification to detect all viral strains.
在一名患有播散性人巨细胞病毒（HCMV）感染的艾滋病患者中，采用目前用于快速鉴定HCMV的HCMV即刻早期（IE）抗原特异性单克隆抗体（MAb）5D2的空斑小室培养法，多次排除血液中存在HCMV，而通过所有其他检测方法（从血液中进行常规病毒分离、抗原血症和病毒血症检测）均反复确认血液中存在HCMV。对该HCMV毒株的序列分析显示，IE基因外显子2存在一个点突变，导致相应蛋白第20位的丝氨酸被脯氨酸取代。对包含该突变的IE基因区域进行克隆和表达表明，这是导致MAb 5D2缺乏反应性的原因。为了检测所有病毒株，必须使用一组IE抗原反应性单克隆抗体而非单一单克隆抗体进行快速HCMV鉴定。
