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Early identification of human cytomegalovirus strains by the shell vial assay is prevented by a novel amino acid substitution in UL123 IE1 gene product.UL123 IE1基因产物中的一种新型氨基酸取代阻碍了通过空斑试验对人巨细胞病毒毒株的早期鉴定。
J Clin Microbiol. 2003 Sep;41(9):4494-5. doi: 10.1128/JCM.41.9.4494-4495.2003.
2
Comparison of monoclonal antibodies for immunostaining in the cytomegalovirus shell vial assay on 4,388 specimens.
J Med Virol. 1995 Nov;47(3):269-71. doi: 10.1002/jmv.1890470313.
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Identification of human cytomegalovirus strain with immediate-early (IE) antigen-specific monoclonal antibody is prevented by point mutation in IE gene.人巨细胞病毒株的即刻早期（IE）抗原特异性单克隆抗体识别可因IE基因中的点突变而受到阻碍。
J Clin Microbiol. 1994 May;32(5):1402-5. doi: 10.1128/jcm.32.5.1402-1405.1994.
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Correlation between human cytomegalovirus quantitative p72 antigenemia and viremia.人巨细胞病毒定量p72抗原血症与病毒血症之间的相关性。
Clin Diagn Virol. 1996 Oct;7(1):63-7. doi: 10.1016/s0928-0197(96)00249-8.
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Transactivation of a human cytomegalovirus early promoter by gene products from the immediate-early gene IE2 and augmentation by IE1: mutational analysis of the viral proteins.人巨细胞病毒早期启动子被即刻早期基因IE2的基因产物反式激活以及被IE1增强：病毒蛋白的突变分析
J Virol. 1990 Apr;64(4):1498-506. doi: 10.1128/JVI.64.4.1498-1506.1990.
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Detection of cytomegalovirus in shell vial cultures by using a DNA probe and early nuclear antigen monoclonal antibody.使用DNA探针和早期核抗原单克隆抗体在空斑小瓶培养物中检测巨细胞病毒。
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Cross-reactivity of HLA-B*1801-restricted T-lymphocyte clones with target cells expressing variants of the human cytomegalovirus 72kDa-IE1 protein.HLA - B*1801限制的T淋巴细胞克隆与表达人巨细胞病毒72kDa - IE1蛋白变体的靶细胞的交叉反应性。
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The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL.人类细胞毒性T淋巴细胞（CTL）对巨细胞病毒的反应以结构蛋白pp65为主导：pp65特异性CTL的频率、特异性和T细胞受体使用情况。
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Human cytomegalovirus proteins pp65 and immediate early protein 1 are common targets for CD8+ T cell responses in children with congenital or postnatal human cytomegalovirus infection.人巨细胞病毒蛋白pp65和立即早期蛋白1是先天性或出生后感染人巨细胞病毒儿童中CD8 + T细胞应答的常见靶标。
J Immunol. 2004 Feb 15;172(4):2256-64. doi: 10.4049/jimmunol.172.4.2256.
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Use of transgenic HLA A*0201/Kb and HHD II mice to evaluate frequency of cytomegalovirus IE1-derived peptide usage in eliciting human CD8 cytokine response.利用转基因 HLA A*0201/Kb 和 HHD II 小鼠评估巨细胞病毒 IE1 衍生肽在引发人 CD8 细胞因子反应中的使用频率。
J Virol. 2003 Apr;77(7):4457-62. doi: 10.1128/jvi.77.7.4457-4462.2003.

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Congenital Human Cytomegalovirus Infection: A Narrative Review of Maternal Immune Response and Diagnosis in View of the Development of a Vaccine and Prevention of Primary and Non-Primary Infections in Pregnancy.先天性人巨细胞病毒感染：鉴于疫苗的研发以及孕期原发性和非原发性感染的预防，对母体免疫反应和诊断的叙述性综述
Microorganisms. 2021 Aug 16;9(8):1749. doi: 10.3390/microorganisms9081749.
2
Fetal human cytomegalovirus transmission correlates with delayed maternal antibodies to gH/gL/pUL128-130-131 complex during primary infection.原发性感染期间母体针对 gH/gL/pUL128-130-131 复合物的抗体出现延迟与胎儿人类巨细胞病毒传播相关。
PLoS One. 2013;8(3):e59863. doi: 10.1371/journal.pone.0059863. Epub 2013 Mar 29.
3
Antibodies against neutralization epitopes of human cytomegalovirus gH/gL/pUL128-130-131 complex and virus spreading may correlate with virus control in vivo.针对人巨细胞病毒 gH/gL/pUL128-130-131 复合物中和表位的抗体和病毒扩散可能与体内病毒控制相关。
J Clin Immunol. 2012 Dec;32(6):1324-31. doi: 10.1007/s10875-012-9739-3. Epub 2012 Jul 27.
4
Oral valganciclovir treatment in newborns with symptomatic congenital cytomegalovirus infection.口服缬更昔洛韦治疗有症状的先天性巨细胞病毒感染的新生儿。
Eur J Clin Microbiol Infect Dis. 2009 Dec;28(12):1465-70. doi: 10.1007/s10096-009-0806-5. Epub 2009 Sep 11.
5
Measurement of the sensitivity of different commercial assays in the diagnosis of CMV infection in pregnancy.不同商业检测方法在妊娠期巨细胞病毒感染诊断中的敏感性测定。
Eur J Clin Microbiol Infect Dis. 2009 Aug;28(8):977-81. doi: 10.1007/s10096-009-0738-0. Epub 2009 Apr 10.

本文引用的文献

1
Diagnosis and management of human cytomegalovirus infection in the mother, fetus, and newborn infant.母亲、胎儿及新生儿人巨细胞病毒感染的诊断与管理
Clin Microbiol Rev. 2002 Oct;15(4):680-715. doi: 10.1128/CMR.15.4.680-715.2002.
2
Identification of human cytomegalovirus strain with immediate-early (IE) antigen-specific monoclonal antibody is prevented by point mutation in IE gene.人巨细胞病毒株的即刻早期（IE）抗原特异性单克隆抗体识别可因IE基因中的点突变而受到阻碍。
J Clin Microbiol. 1994 May;32(5):1402-5. doi: 10.1128/jcm.32.5.1402-1405.1994.
3
Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients.移植患者和艾滋病患者血液白细胞及血浆中人类巨细胞病毒DNA的定量比较
J Clin Microbiol. 1994 Nov;32(11):2709-17. doi: 10.1128/jcm.32.11.2709-2717.1994.
4
Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.通过低速离心和使用针对早期抗原的单克隆抗体，快速检测接种尿液标本的MRC-5细胞中的巨细胞病毒。
J Clin Microbiol. 1984 Jun;19(6):917-9. doi: 10.1128/jcm.19.6.917-919.1984.
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Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins.
J Clin Microbiol. 1990 Dec;28(12):2681-8. doi: 10.1128/jcm.28.12.2681-2688.1990.
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Monoclonal antibody E-13 (M-810) to human cytomegalovirus recognizes an epitope encoded by exon 2 of the major immediate early gene.
J Gen Virol. 1992 Oct;73 ( Pt 10):2699-703. doi: 10.1099/0022-1317-73-10-2699.
7
Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia.不同免疫染色技术和针对低基质磷蛋白（pp65）的单克隆抗体用于人巨细胞病毒抗原血症最佳定量分析的比较。
J Clin Microbiol. 1992 May;30(5):1232-7. doi: 10.1128/jcm.30.5.1232-1237.1992.

Early identification of human cytomegalovirus strains by the shell vial assay is prevented by a novel amino acid substitution in UL123 IE1 gene product.

作者信息

Gerna G, Baldanti F, Percivalle E, Zavattoni M, Campanini G, Revello M G

出版信息

J Clin Microbiol. 2003 Sep;41(9):4494-5. doi: 10.1128/JCM.41.9.4494-4495.2003.

DOI:10.1128/JCM.41.9.4494-4495.2003
PMID:12958307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC193824/
Abstract
摘要