Gleaves C A, Smith T F, Shuster E A, Pearson G R
J Clin Microbiol. 1984 Jun;19(6):917-9. doi: 10.1128/jcm.19.6.917-919.1984.
A commercially available monoclonal antibody directed against an early nuclear protein of cytomegalovirus was used with low-speed centrifugation for the rapid detection of this virus from urine specimens inoculated onto MRC-5 cells. A total of 19 of 162 (11.7%) urine specimens inoculated were positive by both immunofluorescence and peroxidase-antiperoxidase procedures (sensitivity, 100%), whereas only 18 of the samples produced cytopathic effects in conventional cell culture (specificity, 94.7%). All specimens were positive by immunofluorescence and peroxidase-antiperoxidase procedures at 36 h postinfection, whereas an average of 9 days was required for cytopathic effects to develop in cell cultures.
一种针对巨细胞病毒早期核蛋白的市售单克隆抗体，通过低速离心用于从接种到MRC - 5细胞上的尿液标本中快速检测该病毒。在接种的162份尿液标本中，共有19份（11.7%）通过免疫荧光和过氧化物酶 - 抗过氧化物酶方法检测为阳性（灵敏度为100%），而在传统细胞培养中只有18份样本产生细胞病变效应（特异性为94.7%）。所有标本在感染后36小时通过免疫荧光和过氧化物酶 - 抗过氧化物酶方法检测均为阳性，而细胞培养中产生细胞病变效应平均需要9天。
