Pezzone M A, Dohanics J, Rabin B S
Department of Pathology, Brain, Behavior and Immunity Center, Pittsburgh, PA 15213-2582.
J Neuroimmunol. 1994 Aug;53(1):39-46. doi: 10.1016/0165-5728(94)90062-0.
To assess the role of the hypothalamic paraventricular nucleus (PVN) in mediating stressor-induced immune alterations, male Lewis rats were subjected to a 1-h session of intermittent footshock stress or home cage conditions 6 days after receiving bilateral or sham PVN lesions. Splenic and peripheral blood lymphocyte proliferative responses to the non-specific mitogens, concanavalin A (ConA) and phytohemagglutinin (PHA), were subsequently measured as were plasma corticosterone levels. In sham-operated rats, footshock markedly elevated plasma corticosterone levels and concurrently suppressed the proliferative responses of peripheral blood and splenic lymphocytes. In PVN-lesioned rats, however, the shock-induced suppression of lymphocyte proliferation in the peripheral blood and the elevation of plasma corticosterone were significantly attenuated, while lymphocyte proliferation in the spleen was suppressed below the level of the sham-treated animals. Thus, by utilizing ablation studies, we have determined that the PVN may play a direct role in the alteration of lymphocyte function during stress, and an intact PVN buffers the effect of stress on the responsiveness of spleen lymphocytes to non-specific mitogens.
为评估下丘脑室旁核（PVN）在介导应激源诱导的免疫改变中的作用，在雄性Lewis大鼠接受双侧或假PVN损伤6天后，对其进行1小时的间歇性足部电击应激或置于饲养笼对照条件下。随后测量脾和外周血淋巴细胞对非特异性有丝分裂原刀豆蛋白A（ConA）和植物血凝素（PHA）的增殖反应以及血浆皮质酮水平。在假手术大鼠中，足部电击显著提高了血浆皮质酮水平，同时抑制了外周血和脾淋巴细胞的增殖反应。然而，在PVN损伤的大鼠中，电击诱导的外周血淋巴细胞增殖抑制和血浆皮质酮升高显著减弱，而脾淋巴细胞增殖被抑制到低于假手术处理动物的水平。因此，通过利用损毁研究，我们确定PVN可能在应激期间淋巴细胞功能改变中起直接作用，并且完整的PVN可缓冲应激对脾淋巴细胞对非特异性有丝分裂原反应性的影响。
