17β-雌二醇和孕酮对去卵巢猪子宫动脉和子宫肌层中儿茶酚-O-甲基转移酶和单胺氧化酶活性的影响。

Influence of estradiol-17 beta and progesterone on catechol-O-methyltransferase and monoamine oxidase activities in uterine artery and myometrium of ovariectomized pigs.

作者信息

Dynarowicz I, Watkowski T

机构信息

Department of Pharmacology, Faculty of Veterinary Medicine, University of Agriculture and Technology, Olsztyn, Poland.

出版信息

Arch Vet Pol. 1993;33(1-2):29-37.

PMID:8055052

Abstract

Activities of catechol-O-methyltransferase (COMT) and monoamine oxidase (MAO) were determined in uterine artery and myometrium of uterine horn in ovariectomized pigs. It was considered that in uterine artery COMT activity increased after estradiol (E2) by 91.4% (p < 0.01), after progesterone (P) by 194.3% (p < 0.001) and after simultaneous administration of E2+P by 109.3% (p < 0.01). In myometrium this activity rose after E2 by 56.3% (p < 0.01) and after simultaneous using E2+P rose by 64.3% (p < 0.01) in relation to COMT activity determined in ovariectomized animals (Control). MAO activity in uterine artery decreased by 55.4% after P (p < 0.01) and also after simultaneous administration of E2+P by 58.7% (p < 0.01). In myometrium this activity decreased after E2 by 35.5% (p < 0.05) and after E2+P by 51.2% (p < 0.01), whereas increased after P by 30.6% (p < 0.05) in relation to MAO activity determined in control. Changes of MAO and COMT activities after E or P administration in ovariectomized animals denote that the hormones can participate in regulation of NA concentration in adrenergic synapse by the way of qualitative and quantitative changes of this mediator decomposition rate. E2 causes decrease of NA concentration in adrenergic synapse of uterine artery, whereas P operates the opposite way, as increaser.

摘要

测定了去卵巢猪子宫角的子宫动脉和子宫肌层中儿茶酚-O-甲基转移酶（COMT）和单胺氧化酶（MAO）的活性。结果发现，子宫动脉中的COMT活性在给予雌二醇（E2）后增加了91.4%（p<0.01），给予孕酮（P）后增加了194.3%（p<0.001），同时给予E2+P后增加了109.3%（p<0.01）。在子宫肌层中，与去卵巢动物（对照）中测定的COMT活性相比，E2后该活性增加了56.3%（p<0.01），同时使用E2+P后增加了64.3%（p<0.01）。子宫动脉中的MAO活性在给予P后降低了55.4%（p<0.01），同时给予E2+P后也降低了58.7%（p<0.01）。在子宫肌层中，与对照中测定的MAO活性相比，E2后该活性降低了35.5%（p<0.05），E2+P后降低了51.2%（p<0.01），而P后增加了30.6%（p<0.05）。去卵巢动物给予E或P后MAO和COMT活性的变化表明，这些激素可通过改变这种介质分解速率的质和量来参与调节肾上腺素能突触中去甲肾上腺素（NA）的浓度。E2导致子宫动脉肾上腺素能突触中NA浓度降低，而P则相反，起增加作用。