Porta M, Molinatti P A, Dosso A A, Williams F M, Brooks R A, Kohner E M
Diabetic Retinopathy Unit, Hammersmith Hospital, London, United Kingdom.
Diabete Metab. 1994 Jan-Feb;20(1):25-30.
Selective loss of capillary pericytes occurs early in diabetic and galactosemic retinopathies, at a stage when endothelial cells are still spared. To ascertain whether such loss is directly related to high hexose concentrations, the replication of bovine retinal pericytes and endothelial cells was studied by culturing them in media containing 5.6 mmol/l glucose alone and enriched with extra glucose, mannitol or galactose to achieve final concentrations of 16.7, 27.8 and 50.0 mmol/l. At the end of the incubation there were significantly less pericytes/culture cell in 16.7 mmol/l glucose (341 +/- 78 x 10(3), mean +/- SEM, p = 0.027) and galactose (304 +/- 55 x 10(3), p = 0.046) than in 5.6 mmol/l glucose (417 +/- 98 and 355 +/- 75 x 10(3) for separate experiments, respectively). Mannitol had no such effect (343 +/- 52 vs. 337 +/- 52 x 10(3). Endothelial cells did not change in number, except in 50.0 mmol/l glucose (533 +/- 66 vs. 629 +/- 67 x 10(3) at 5.6 mmol/l; p = 0.026) and 50.0 mmol/l galactose (440 +/- 48 vs. 592 +/- 51 x 10(3); p = 0.013). To verify if these effects are due to decreased replication, the uptake of 3H-thymidine was measured in pericytes and endothelial cells subjected to the same concentrations of glucose, mannitol and galactose. In pericytes, thymidine uptake was reduced in the presence of 16.7 mmol/l glucose (p = 0.003, compared to 5.6 mmol/l) and galactose (p = 0.027) but not mannitol. Pericyte counts and thymidine uptake was reduced for concentrations of glucose, galactose and mannitol higher than 16.7 mmol/l.(ABSTRACT TRUNCATED AT 250 WORDS)
在糖尿病性和半乳糖血症性视网膜病变的早期，当内皮细胞尚未受到损害时，毛细血管周细胞就会出现选择性丢失。为了确定这种丢失是否与高己糖浓度直接相关，通过将牛视网膜周细胞和内皮细胞培养在仅含5.6 mmol/l葡萄糖以及添加额外葡萄糖、甘露醇或半乳糖以达到最终浓度为16.7、27.8和50.0 mmol/l的培养基中，研究了它们的增殖情况。培养结束时，与5.6 mmol/l葡萄糖（分别进行的实验中为417 +/- 98和355 +/- 75 x 10(3)）相比，16.7 mmol/l葡萄糖（341 +/- 78 x 10(3)，平均值 +/- 标准误，p = 0.027）和半乳糖（304 +/- 55 x 10(3)，p = 0.046）中的周细胞/培养细胞明显减少。甘露醇没有这种作用（343 +/- 52与337 +/- 52 x 10(3)）。内皮细胞数量没有变化，除了在50.0 mmol/l葡萄糖（5.6 mmol/l时为533 +/- 66与629 +/- 67 x 10(3)；p = 0.026）和50.0 mmol/l半乳糖（440 +/- 48与592 +/- 51 x 10(3)；p = 0.013）中。为了验证这些影响是否由于复制减少所致，在处于相同葡萄糖、甘露醇和半乳糖浓度的周细胞和内皮细胞中测量了3H - 胸腺嘧啶核苷的摄取。在周细胞中，16.7 mmol/l葡萄糖（与5.6 mmol/l相比，p = 0.003）和半乳糖（p = 0.027）存在时胸腺嘧啶核苷摄取减少，但甘露醇不存在这种情况。对于高于16.7 mmol/l的葡萄糖、半乳糖和甘露醇浓度，周细胞计数和胸腺嘧啶核苷摄取减少。（摘要截断于250字）
