Ogawa T, Mori H, Yasuda K, Hasegawa M
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Suita-Osaka, Japan.
FEMS Microbiol Lett. 1994 Jul 1;120(1-2):23-30. doi: 10.1111/j.1574-6968.1994.tb07002.x.
A 72-kDa major cell-surface protein (72K-CSP) was purified from the wash fluid of Porphyromonas gingivalis OMZ409. Using the synthetic oligonucleotide probes corresponding to the determined amino-terminal amino acid sequence of 72K-CSP, recombinant plasmid clones carrying approx. 3.4-kb KpnI-XhoI fragments in XL1-Blue libraries of P. gingivalis OMZ409 and 381 were obtained. The premature form proteins of 558 and 563 amino acids led by putative signal sequences were thought to be processed to form the mature proteins of a predicted size of 55,655 Da for strain OMZ409 and of 55,654 for strain 381. Both proteins had unusual proline-rich regions in their carboxyl-terminal regions. No homologous sequences could be found in protein databases. Examination of antigen-specific antibody responses in the serum of patients with adult periodontitis by ELISA revealed that 72K-CSP had a different immunoreactivity from that of P. gingivalis 381 fimbriae.
从牙龈卟啉单胞菌OMZ409的洗涤液中纯化出一种72千道尔顿的主要细胞表面蛋白（72K-CSP）。使用与已确定的72K-CSP氨基末端氨基酸序列相对应的合成寡核苷酸探针，在牙龈卟啉单胞菌OMZ409和381的XL1-Blue文库中获得了携带约3.4千碱基KpnI-XhoI片段的重组质粒克隆。由推定信号序列引导的含558和563个氨基酸的前体蛋白被认为经过加工形成预测大小为55,655道尔顿的菌株OMZ409的成熟蛋白和55,654道尔顿的菌株381的成熟蛋白。两种蛋白在其羧基末端区域都有不寻常的富含脯氨酸的区域。在蛋白质数据库中未发现同源序列。通过ELISA检测成人牙周炎患者血清中的抗原特异性抗体反应，发现72K-CSP与牙龈卟啉单胞菌381菌毛具有不同的免疫反应性。
