Brickner J H, Lynch T J, Zeilinger D, Orias E
Department of Biological Sciences, University of California, Santa Barbara 93106, USA.
Genetics. 1996 Jun;143(2):811-21. doi: 10.1093/genetics/143.2.811.
Using the random amplified polymorphic DNA (RAPD) technique and exploiting the unique genetics of Tetrahymena thermophila, we have identified and characterized 40 DNA polymorphisms occurring between two inbred strains (B and C3) of this ciliated protozoan. These RAPD markers permit the PCR amplification of a DNA species using template DNA from SB1969 (B strain) but fail to do so using DNA from C3-368-5 (C3 strain). Polymorphisms were mapped to chromosomes using a panel of monosomic strains constructed by crossing B strain-derived nullisomic strains to inbred strain C3. They map to all five chromosomes and appear to be evenly distributed throughout the genome. Chromosomal groups were then analyzed for linkage using meiotic segregants; four linkage groups were identified in chromosomes 1R 2L, 3 and 5. The RAPD method appears useful for the construction of a genetic map of the Tetrahymena genome based on DNA polymorphisms.
利用随机扩增多态性DNA（RAPD）技术，并借助嗜热四膜虫独特的遗传学特性，我们已鉴定并表征了在这种纤毛原生动物的两个近交系（B和C3）之间出现的40个DNA多态性。这些RAPD标记允许使用来自SB1969（B系）的模板DNA进行DNA物种的PCR扩增，但使用来自C3 - 368 - 5（C3系）的DNA则无法进行扩增。利用通过将B系衍生的缺体菌株与近交系C3杂交构建的一组单体菌株，将多态性定位到染色体上。它们定位于所有五条染色体，并且似乎在整个基因组中均匀分布。然后使用减数分裂分离子分析染色体组的连锁情况；在染色体1R、2L、3和5中鉴定出四个连锁群。基于DNA多态性，RAPD方法似乎有助于构建嗜热四膜虫基因组的遗传图谱。
