嗜热四膜虫随机扩增多态性DNA的鉴定、定位及连锁分析
Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in Tetrahymena thermophila.
作者信息
Brickner J H, Lynch T J, Zeilinger D, Orias E
机构信息
Department of Biological Sciences, University of California, Santa Barbara 93106, USA.
出版信息
Genetics. 1996 Jun;143(2):811-21. doi: 10.1093/genetics/143.2.811.
Abstract
Using the random amplified polymorphic DNA (RAPD) technique and exploiting the unique genetics of Tetrahymena thermophila, we have identified and characterized 40 DNA polymorphisms occurring between two inbred strains (B and C3) of this ciliated protozoan. These RAPD markers permit the PCR amplification of a DNA species using template DNA from SB1969 (B strain) but fail to do so using DNA from C3-368-5 (C3 strain). Polymorphisms were mapped to chromosomes using a panel of monosomic strains constructed by crossing B strain-derived nullisomic strains to inbred strain C3. They map to all five chromosomes and appear to be evenly distributed throughout the genome. Chromosomal groups were then analyzed for linkage using meiotic segregants; four linkage groups were identified in chromosomes 1R 2L, 3 and 5. The RAPD method appears useful for the construction of a genetic map of the Tetrahymena genome based on DNA polymorphisms.
摘要
利用随机扩增多态性DNA（RAPD）技术，并借助嗜热四膜虫独特的遗传学特性，我们已鉴定并表征了在这种纤毛原生动物的两个近交系（B和C3）之间出现的40个DNA多态性。这些RAPD标记允许使用来自SB1969（B系）的模板DNA进行DNA物种的PCR扩增，但使用来自C3 - 368 - 5（C3系）的DNA则无法进行扩增。利用通过将B系衍生的缺体菌株与近交系C3杂交构建的一组单体菌株，将多态性定位到染色体上。它们定位于所有五条染色体，并且似乎在整个基因组中均匀分布。然后使用减数分裂分离子分析染色体组的连锁情况；在染色体1R、2L、3和5中鉴定出四个连锁群。基于DNA多态性，RAPD方法似乎有助于构建嗜热四膜虫基因组的遗传图谱。
相似文献
1
Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in Tetrahymena thermophila.嗜热四膜虫随机扩增多态性DNA的鉴定、定位及连锁分析
Genetics. 1996 Jun;143(2):811-21. doi: 10.1093/genetics/143.2.811.
2
Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of Tetrahymena thermophila.与嗜热四膜虫交配型位点紧密连锁的随机扩增多态性DNA的遗传图谱。
Genetics. 1995 Dec;141(4):1315-25. doi: 10.1093/genetics/141.4.1315.
3
Mapping three classical isozyme loci in tetrahymena: meiotic linkage of EstA to the ChxA linkage group.在四膜虫中定位三个经典同工酶基因座：酯酶A与ChxA连锁群的减数分裂连锁
Genetics. 1996 Dec;144(4):1489-96. doi: 10.1093/genetics/144.4.1489.
4
A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.一种利用聚合酶链反应绘制嗜热四膜虫种系序列图谱的方法。
Genetics. 1994 May;137(1):95-106. doi: 10.1093/genetics/137.1.95.
5
Genome-wide characterization of Tetrahymena thermophila chromosome breakage sites. II. Physical and genetic mapping.嗜热四膜虫染色体断裂位点的全基因组特征分析。II. 物理和遗传图谱分析。
Genetics. 2005 Aug;170(4):1623-31. doi: 10.1534/genetics.104.031435. Epub 2005 Jun 14.
6
Autonomously replicating macronuclear DNA pieces are the physical basis of genetic coassortment groups in Tetrahymena thermophila.自主复制的大核DNA片段是嗜热四膜虫中基因共分类群体的物理基础。
Genetics. 2000 Jul;155(3):1119-25. doi: 10.1093/genetics/155.3.1119.
7
Identification and genetic mapping of random amplified polymorphic DNA (RAPD) markers to the sheep genome.随机扩增多态性DNA（RAPD）标记在绵羊基因组中的鉴定与遗传定位。
Mamm Genome. 1996 Aug;7(8):580-5. doi: 10.1007/s003359900173.
8
Tetrahymena thermophila genetics: concepts and applications.嗜热四膜虫遗传学：概念与应用
Methods Cell Biol. 2012;109:301-25. doi: 10.1016/B978-0-12-385967-9.00010-4.
9
Tetrahymena macronuclear genome mapping: colinearity Of macronuclear coassortment groups and the micronuclear map on chromosome 1l.嗜热四膜虫大核基因组图谱绘制：大核共分配组与第11号染色体上的小核图谱的共线性
Genetics. 2000 Mar;154(3):1155-67. doi: 10.1093/genetics/154.3.1155.
10
Tetrahymena micronuclear genome mapping. a high-resolution meiotic map of chromosome 1l.四膜虫小核基因组图谱。第11号染色体的高分辨率减数分裂图谱。
Genetics. 2000 Mar;154(3):1141-53. doi: 10.1093/genetics/154.3.1141.
引用本文的文献
1
Tetrahymena as a Unicellular Model Eukaryote: Genetic and Genomic Tools.作为单细胞模式真核生物的四膜虫：遗传和基因组工具
Genetics. 2016 Jun;203(2):649-65. doi: 10.1534/genetics.114.169748.
2
Tetrahymena in the laboratory: strain resources, methods for culture, maintenance, and storage.实验室中的四膜虫：菌株资源、培养、保存及储存方法
Methods Cell Biol. 2012;109:237-76. doi: 10.1016/B978-0-12-385967-9.00008-6.
3
Tetrahymena thermophila, a unicellular eukaryote with separate germline and somatic genomes.嗜热四膜虫，一种具有独立生殖系和体细胞基因组的单细胞真核生物。
Res Microbiol. 2011 Jul-Aug;162(6):578-86. doi: 10.1016/j.resmic.2011.05.001. Epub 2011 May 18.
4
Genome-wide characterization of Tetrahymena thermophila chromosome breakage sites. II. Physical and genetic mapping.嗜热四膜虫染色体断裂位点的全基因组特征分析。II. 物理和遗传图谱分析。
Genetics. 2005 Aug;170(4):1623-31. doi: 10.1534/genetics.104.031435. Epub 2005 Jun 14.
5
Genetic linkage map of the edible basidiomycete Pleurotus ostreatus.食用担子菌平菇的遗传连锁图谱。
Appl Environ Microbiol. 2000 Dec;66(12):5290-300. doi: 10.1128/AEM.66.12.5290-5300.2000.
6
Autonomously replicating macronuclear DNA pieces are the physical basis of genetic coassortment groups in Tetrahymena thermophila.自主复制的大核DNA片段是嗜热四膜虫中基因共分类群体的物理基础。
Genetics. 2000 Jul;155(3):1119-25. doi: 10.1093/genetics/155.3.1119.
7
Tetrahymena macronuclear genome mapping: colinearity Of macronuclear coassortment groups and the micronuclear map on chromosome 1l.嗜热四膜虫大核基因组图谱绘制：大核共分配组与第11号染色体上的小核图谱的共线性
Genetics. 2000 Mar;154(3):1155-67. doi: 10.1093/genetics/154.3.1155.
8
Tetrahymena micronuclear genome mapping. a high-resolution meiotic map of chromosome 1l.四膜虫小核基因组图谱。第11号染色体的高分辨率减数分裂图谱。
Genetics. 2000 Mar;154(3):1141-53. doi: 10.1093/genetics/154.3.1141.
9
Chlamydomonas: the cell and its genomes.衣藻：细胞及其基因组。
Genetics. 1999 Jan;151(1):9-14. doi: 10.1093/genetics/151.1.9.
10
A dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers.基于1018个分子标记构建的覆盖家蚕所有染色体的高密度遗传图谱。
Genetics. 1998 Dec;150(4):1513-25. doi: 10.1093/genetics/150.4.1513.
本文引用的文献
1
Linkage and Genetic Map Length in TETRAHYMENA THERMOPHILA.四膜虫中的连锁与遗传图谱长度。
Genetics. 1977 Nov;87(3):421-39. doi: 10.1093/genetics/87.3.421.
2
Nullisomic Tetrahymena. II. a Set of Nullisomics Define the Germinal Chromosomes.单体型四膜虫。二。一组单体型定义了生殖染色体。
Genetics. 1983 Jun;104(2):257-70. doi: 10.1093/genetics/104.2.257.
3
LINKAGE STUDIES IN VARIETY 1 OF TETRAHYMENA PYRIFORMIS: A FIRST CASE OF LINKAGE IN THE CILIATED PROTOZOA.梨形四膜虫1号变种的连锁研究：纤毛原生动物中连锁的首例。
Genetics. 1964 Apr;49(4):617-27. doi: 10.1093/genetics/49.4.617.
4
Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of Tetrahymena thermophila.与嗜热四膜虫交配型位点紧密连锁的随机扩增多态性DNA的遗传图谱。
Genetics. 1995 Dec;141(4):1315-25. doi: 10.1093/genetics/141.4.1315.
5
Genetic analysis with random amplified polymorphic DNA markers.利用随机扩增多态性DNA标记进行遗传分析。
Plant Physiol. 1993 Feb;101(2):349-52. doi: 10.1104/pp.101.2.349.
6
A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.一种利用聚合酶链反应绘制嗜热四膜虫种系序列图谱的方法。
Genetics. 1994 May;137(1):95-106. doi: 10.1093/genetics/137.1.95.
7
The 5S ribosomal RNA gene clusters in Tetrahymena thermophila: strain differences, chromosomal localization, and loss during micronuclear ageing.嗜热四膜虫中的5S核糖体RNA基因簇：菌株差异、染色体定位及小核衰老过程中的丢失
Mol Gen Genet. 1984;197(2):244-53. doi: 10.1007/BF00330970.
8
C-terminal amidation of neuropeptides. Gly-Lys-Arg extension an efficient precursor of C-terminal amide.神经肽的C末端酰胺化。甘氨酸-赖氨酸-精氨酸延伸是C末端酰胺的有效前体。
FEBS Lett. 1984 Feb 13;167(1):160-4. doi: 10.1016/0014-5793(84)80853-4.
9
Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena.自我剪接RNA：嗜热四膜虫核糖体RNA间隔序列的自动切除与自动环化
Cell. 1982 Nov;31(1):147-57. doi: 10.1016/0092-8674(82)90414-7.
10
Cytogenetics of genomic exclusion in Tetrahymena.四膜虫基因组排除的细胞遗传学
Genetics. 1967 Apr;55(4):797-822. doi: 10.1093/genetics/55.4.797.
Zotero 插件