Vaughan J A, Noden B H, Beier J C
Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205.
J Med Entomol. 1994 May;31(3):445-9. doi: 10.1093/jmedent/31.3.445.
We examined the relative susceptibilities of Anopheles gambiae Giles of different physiological ages to infection with cultured Plasmodium falciparum (Welch). Cohorts of mosquitoes were divided into three groups; one was fed uninfected blood on day 3 after emergence (i.e., one prior blood meal); another on days 3 and 7 after emergence (i.e., two prior blood meals); and a control group was maintained on sucrose. On days 10 to 12 after emergence, mosquitoes were fed human blood containing P. falciparum gametocytes. Prior blood feeding accelerated digestion of the infective blood meals and subtly altered susceptibility to infection with P. falciparum. When gametocyte cultures were highly fertile, all experimental groups were equally susceptible to infection. However, when gametocyte fertility was low, accelerated digestion had a detrimental effect on the transition of ookinetes to oocysts. Accelerated digestion may raise the threshold density of ookinetes required for the successful conversion of ookinetes to oocysts.
我们研究了不同生理年龄的冈比亚按蚊对培养的恶性疟原虫（韦尔奇）感染的相对易感性。将蚊群分为三组；一组在羽化后第3天喂食未感染的血液（即一次先前的血餐）；另一组在羽化后第3天和第7天喂食（即两次先前的血餐）；对照组以蔗糖维持。在羽化后第10至12天，给蚊子喂食含有恶性疟原虫配子体的人血。先前的血餐加速了感染性血餐的消化，并微妙地改变了对恶性疟原虫感染的易感性。当配子体培养物高度 fertile 时，所有实验组对感染的易感性相同。然而，当配子体 fertility 较低时，加速消化对动合子向卵囊的转变有不利影响。加速消化可能会提高动合子成功转化为卵囊所需的动合子阈值密度。
