Newcomb W W, Homa F L, Thomsen D R, Ye Z, Brown J C
Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville 22908.
J Virol. 1994 Sep;68(9):6059-63. doi: 10.1128/JVI.68.9.6059-6063.1994.
Herpes simplex virus type 1 (HSV-1) capsids were found to assemble spontaneously in a cell-free system consisting of extracts prepared from insect cells that had been infected with recombinant baculoviruses coding for HSV-1 capsid proteins. The capsids formed in this system resembled native HSV-1 capsids in morphology as judged by electron microscopy, in sedimentation rate on sucrose density gradients, in protein composition, and in their ability to react with antibodies specific for the HSV-1 major capsid protein, VP5. Optimal capsid assembly required the presence of extracts containing capsid proteins VP5, VP19, VP23, VP22a, and the maturational protease (product of the UL26 gene). Assembly was more efficient at 27 degrees C than at 4 degrees C. The availability of a cell-free assay for HSV-1 capsid formation will be of help in identifying the morphogenetic steps that occur during capsid assembly in vivo and in evaluating candidate antiherpes therapeutics directed at capsid assembly.
在一个无细胞系统中发现，单纯疱疹病毒1型（HSV-1）衣壳能够自发组装。该无细胞系统由昆虫细胞提取物组成，这些昆虫细胞已被编码HSV-1衣壳蛋白的重组杆状病毒感染。通过电子显微镜判断，此系统中形成的衣壳在形态上类似于天然HSV-1衣壳，在蔗糖密度梯度中的沉降速率、蛋白质组成以及与HSV-1主要衣壳蛋白VP5特异性抗体反应的能力方面也相似。最佳衣壳组装需要存在含有衣壳蛋白VP5、VP19、VP23、VP22a和成熟蛋白酶（UL26基因产物）的提取物。在27摄氏度时组装比在4摄氏度时更有效。HSV-1衣壳形成的无细胞检测方法将有助于确定体内衣壳组装过程中发生的形态发生步骤，并有助于评估针对衣壳组装的候选抗疱疹治疗药物。
