Murakami S, Arai Y
Department of Anatomy, Juntendo University School of Medicine, Tokyo, Japan.
Neurosci Res. 1994 May;19(3):331-8. doi: 10.1016/0168-0102(94)90046-9.
To examine the possible migration of luteinizing hormone-releasing hormone (LHRH) neurons from the olfactory placode to the brain, the epithelial cells of the olfactory placode of the chick embryos were labeled with a fluorescent carbocyanine dye DiI at embryonic days 3.5-4.0. The embryos treated with DiI were incubated in ovo for 1 to 7 days. The DiI-labeled cells were first detected in the olfactory epithelium and olfactory nerve one day after the application of DiI. Two to four days after the application of the dye, the labeled cells sequentially appeared in the rostral and medial forebrain, and in the septo-preoptic area. The distribution pattern of DiI-labeled cells closely resembled that of LHRH neurons. Double staining for DiI and LHRH demonstrated that DiI-labeled cells co-expressed LHRH. However, there were a number of DiI-labeled cells which did not co-express LHRH. These results provide evidence for the actual migration of LHRH neurons from the olfactory placode to the septo-preoptic area during the development, and also suggest that some non-LHRH neurons in the forebrain are of olfactory placodal origin.
为了研究促黄体生成激素释放激素（LHRH）神经元从嗅基板向脑内的可能迁移情况，在胚胎第3.5至4.0天，用荧光碳氰染料DiI对鸡胚嗅基板的上皮细胞进行标记。用DiI处理过的胚胎在卵内孵育1至7天。在应用DiI一天后，首先在嗅上皮和嗅神经中检测到DiI标记的细胞。在应用染料后的2至4天，标记细胞依次出现在前脑的 Rostral 和内侧以及隔区 - 视前区。DiI标记细胞的分布模式与LHRH神经元的分布模式非常相似。对DiI和LHRH进行双重染色表明，DiI标记的细胞共表达LHRH。然而，有许多DiI标记的细胞不共表达LHRH。这些结果为发育过程中LHRH神经元从嗅基板向隔区 - 视前区的实际迁移提供了证据，也表明前脑中的一些非LHRH神经元起源于嗅基板。
