Krahenbuhl J L, Lambert L H
J Natl Cancer Inst. 1975 Jun;54(6):1433-7. doi: 10.1093/jnci/54.6.1433.
The kinetics of the in vitro interaction between activated mouse macrophages and tumor target cells were studied in Swiss-Webster female mice with the use of the inhibition of uptake of tritiated thymidine by target cells as a quantitative measure of macrophage-effected cytostasis. Unlike the normal macrophage, the individual activated macrophage had an enhanced capacity to inhibit DNA synthesis in tumor target cells. Such cytostasis appeared to result from direct contact between activated macrophages and target cells. Culture conditions discouraging direct contact caused little or no cytostasis, regardless of the ratio of macrophages to target cells. When culture conditions favored direct contact between effector cells and target cells, cytostasis was markedly increased if the ratio of macrophages to target cells was raised.
利用靶细胞对氚标记胸腺嘧啶核苷摄取的抑制作为巨噬细胞介导的细胞生长停滞的定量指标，研究了瑞士-韦伯斯特雌性小鼠体内活化的小鼠巨噬细胞与肿瘤靶细胞之间的体外相互作用动力学。与正常巨噬细胞不同，单个活化的巨噬细胞抑制肿瘤靶细胞DNA合成的能力增强。这种细胞生长停滞似乎是由活化的巨噬细胞与靶细胞之间的直接接触引起的。无论巨噬细胞与靶细胞的比例如何，不利于直接接触的培养条件几乎不会引起细胞生长停滞或根本不会引起细胞生长停滞。当培养条件有利于效应细胞与靶细胞之间的直接接触时，如果提高巨噬细胞与靶细胞的比例，细胞生长停滞会显著增加。
