Malek A, Sager R, Eckardt K U, Bauer C, Schneider H
Department of Obstetrics and Gynecology, University of Berne, Switzerland.
Pflugers Arch. 1994 May;427(1-2):157-61. doi: 10.1007/BF00585955.
The transfer of human recombinant erythropoietin (rhEPO) from the maternal to the fetal side was investigated using the technique of in vitro perfusion of an isolated cotyledon of human placenta, with recirculation of the perfusate (130 ml) in separate closed maternal and fetal circuits. rhEPO (221-512 U), together with [14C]BSA (bovine serum albumin, 44.8 kBq or 2,688,000 dpm), was added to the maternal circuit only. Despite a considerably lower molecular weight of EPO (mol. wt. = 30,400 Da) compared to BSA (mol. wt. = 69,000 Da), no difference was found in their transfer across the placenta from the maternal to the fetal side, which was very low for both macromolecules. The total transfer of rhEPO derived from the concentration measured in the samples taken from the fetal circuit at the end of 4-5 h of perfusion, was in the range of 0.04% of the amount initially added to the maternal compartment. A similar amount of transfer was determined for [14C]BSA (0.04-0.07%, n = 12). In conclusion, by direct determination in a dually in vitro perfused human placental cotyledon, no significant transfer of rhEPO from the maternal to the fetal side could be shown.
采用体外灌注人胎盘分离叶的技术，在单独封闭的母体和胎儿循环中使灌注液（130毫升）再循环，研究了重组人促红细胞生成素（rhEPO）从母体向胎儿侧的转运。仅在母体循环中加入rhEPO（221 - 512单位）和[14C]牛血清白蛋白（BSA，44.8千贝克勒尔或2,688,000每分钟衰变数）。尽管与BSA（分子量 = 69,000道尔顿）相比，EPO的分子量（分子量 = 30,400道尔顿）低得多，但二者从母体向胎儿侧穿过胎盘的转运并无差异，且两种大分子的转运都非常低。根据灌注4 - 5小时结束时从胎儿循环采集的样本中测得的浓度计算，rhEPO的总转运量在最初添加到母体隔室的量的0.04%范围内。[14C]BSA的转运量与之相似（0.04 - 0.07%，n = 12）。总之，通过在双体外灌注的人胎盘叶中直接测定，未显示rhEPO从母体向胎儿侧有明显转运。
