Casali A M, Siringo S, Sofia S, Bolondi L, Di Febo G, Cavalli G
Institute of Histology and General Embryology, University of Genova, Italy.
Pathol Res Pract. 1994 Feb;190(2):201-6. doi: 10.1016/S0344-0338(11)80712-0.
The volume density of bile ducts in adult normal liver has been analyzed in order to provide stereometric parameters to which refer on performing quantitative evaluations of bile duct loss in acquired liver diseases. Five livers were studied by applying a semi-automatic image analysis system (ASM 68K Leitz) to histological sections. No significant differences (P > 0.10) were found for all measures and the following values were derived from the pooled data: (1) mean % volume of bile ducts in liver = 0.318 +/- 0.171; (2) mean % volume of portal tracts in liver = 4.351 +/- 2.860; (3) mean % volume of bile ducts in portal tracts = 6.567 +/- 3.813. The parallelism of bile duct to arterial components of portal tracts was also investigated and expressed as the ratio of their respective volume fractions (mean ratio = 1.72). The validity of the obtained parameters was tested by comparing them with values determined in five cases of primary biliary cirrhosis (PBC). All PBC cases showed a marked decrease in both bile duct % volume in liver (ranging between 0.028 and 0.057) and bile duct % volume in portal tracts (ranging between 0.673 and 0.914), as well as inversion of the bile duct to artery volume ratio in portal tracts (ranging between 0.246 and 0.437).
为了提供立体测量参数，以便在对获得性肝病中的胆管损失进行定量评估时作为参考，对成年正常肝脏中胆管的体积密度进行了分析。通过将半自动图像分析系统（ASM 68K Leitz）应用于组织学切片，对五例肝脏进行了研究。所有测量结果均未发现显著差异（P>0.10），以下值来自汇总数据：（1）肝脏中胆管的平均体积百分比=0.318±0.171；（2）肝脏中门静脉区域的平均体积百分比=4.351±2.860；（3）门静脉区域中胆管的平均体积百分比=6.567±3.813。还研究了胆管与门静脉区域动脉成分的平行性，并将其表示为各自体积分数的比值（平均比值=1.72）。通过将获得的参数与五例原发性胆汁性肝硬化（PBC）病例中确定的值进行比较，测试了所获参数的有效性。所有PBC病例均显示肝脏中胆管体积百分比（范围在0.028至0.057之间）和门静脉区域中胆管体积百分比（范围在0.673至0.914之间）均显著降低，以及门静脉区域中胆管与动脉体积比倒置（范围在0.246至0.437之间）。
